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Field studies on viral diseases affecting farm ruminants / Ahmed Zaki Anwer ; Supervised Hassan Mohmmed Elsaid , Amr Abdelaziz , Iman Bastweecy

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Ahmed Zaki Anwer , 2015Description: 143 P. : charts , photographs ; 25cmOther title:
  • دراسات حقلية للأمراض الفيروسية التى تصيب المجترات فى المزرعة [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases Summary: Malignant catarrhal fever (MCF) is a lymphoproliferative multisystemic fatal syndrome of many domestic and wild animals. Two forms of MCF were originally described, the sheep associated type (SA - MCF) is the most popular one. Due to the common clinical signs between the skin form of MCF and lumpy skin disease, accurate differential diagnosis is required to discriminate between them and to confirm their clinical occurrence. The present study was conducted on 169 animals of different species (130 cattle, 8 buffaloes, 31 sheep). The clinical examination revealed high persistent fever, corneal opacity, mucopurulent oronasal discharges, mucosal lesions and ulcerative skin lesions. All of these symptoms have been observed in the clinical cases of MCF and LSD. No clinical signs were observed in sheep. Peripheral blood leukocytes (Pbl) samples (169) were collected for laboratory investigation. Semi nested PCR and simple conventional PCR assays have been used for MCF and LSD respectively, sequencing and phylogenetic analysis were performed for the positive PCR products. For the MCF cases, further confirmatory diagnostic assays as histopathology and transmission electron microscopy (TEM) were also performed. OvHV - 2 DNA was detected in PBL of 25 animals (12 cattle, 2 buffaloes and 11 sheep). The phylogenetic analysis of the OvH - 2 PCR products revealed 100% identity with the OVH - 2 strains of Brazil, USA and India. The histopathological examination showed pansystemic vasculitis with lymphocytic infiltration in lymphoid and non lymphoid organs. The PCR results of LSD revealed 70 case are positive (all of them were cattle) there was no incidence of LSDV in the examined buffaloes. Sequence analysis for LSDV PCR product the revealed that the prevalent virus isolate among the Egyptian cattle is the LSD Ismailia / 89 strain
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.09.M.Sc.2015.Ah.F (Browse shelf(Opens below)) Not for loan 01010110069090000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.09.M.Sc.2015.Ah.F (Browse shelf(Opens below)) 69090.CD Not for loan 01020110069090000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases

Malignant catarrhal fever (MCF) is a lymphoproliferative multisystemic fatal syndrome of many domestic and wild animals. Two forms of MCF were originally described, the sheep associated type (SA - MCF) is the most popular one. Due to the common clinical signs between the skin form of MCF and lumpy skin disease, accurate differential diagnosis is required to discriminate between them and to confirm their clinical occurrence. The present study was conducted on 169 animals of different species (130 cattle, 8 buffaloes, 31 sheep). The clinical examination revealed high persistent fever, corneal opacity, mucopurulent oronasal discharges, mucosal lesions and ulcerative skin lesions. All of these symptoms have been observed in the clinical cases of MCF and LSD. No clinical signs were observed in sheep. Peripheral blood leukocytes (Pbl) samples (169) were collected for laboratory investigation. Semi nested PCR and simple conventional PCR assays have been used for MCF and LSD respectively, sequencing and phylogenetic analysis were performed for the positive PCR products. For the MCF cases, further confirmatory diagnostic assays as histopathology and transmission electron microscopy (TEM) were also performed. OvHV - 2 DNA was detected in PBL of 25 animals (12 cattle, 2 buffaloes and 11 sheep). The phylogenetic analysis of the OvH - 2 PCR products revealed 100% identity with the OVH - 2 strains of Brazil, USA and India. The histopathological examination showed pansystemic vasculitis with lymphocytic infiltration in lymphoid and non lymphoid organs. The PCR results of LSD revealed 70 case are positive (all of them were cattle) there was no incidence of LSDV in the examined buffaloes. Sequence analysis for LSDV PCR product the revealed that the prevalent virus isolate among the Egyptian cattle is the LSD Ismailia / 89 strain

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