header
Image from OpenLibrary

The immunomodulatory effect of umbilical cord blood mesenchymal stem cells on peripheral blood lymphocytes invitro / Hadeel Hosny Abdellatif ; Supervised Shereen Mahmoud Kamel , Ahmed Zakaria Elshika , Engy Mohamed Elkhatib

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hadeel Hosny Abdellatif , 2015Description: 146 P. : facsimiles ; 25cmOther title:
  • تأثير التعديل المناعى لخلايا المزنكيوم الجذعية المستخلصة من دم الحبل السرى على الخلايا الليمفاوية خارج الجسم [Added title page title]
Subject(s): Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Umbilical cord blood (UCB) gained high interest as a source of stem cells for use in cellular therapies. The immune modulatory effect of mesenchymal stem cells (MSCs) originating from bone marrow, adipose tissue and amniotic membrane was previously proved. In this study we aimed at isolation of MSCs from UCB, and evaluate their immune-modulatory effect on peripheral blood lymphocytes proliferation by two different techniques; 5- Bromo - 2- deoxyuridine (Brdu) enzyme linked immunsorbant assay (ELISA) and carboxy Fluorescine diacetate Succinimidyl Ester (CFSE) flow cytometric technique. MSCs were isolated from UCB, propagated until passage 4, characterized for cell surface markers by flow cytometry and ability of mesodermal differentiation was induced towards osteocytes and adipocytes. differentiation was induced towards osteocytes and adipocytes. The immunosuppressive effect on peripheral blood lymphocytes was examined by co - culturing mitomycin C treated UCB MSCs with mitogen-stimulated lymphocytes for 72 hours. Thereafter, proliferation of lymphocytes was detected by CFSE flow cytometry and colorimetric ELISA. The level of cytokines in cell culture supernatant was also assayed to detect the possible mechanism of immunomodulation. UCB MSCs were able to suppress mitogen-stimulated lymphocytes proliferation; this is due to cell-cell contact as well as due to cytokines secretion. The level of transforming growth factor beta (TGFÝ) and interleukin (IL) 10 increased and the level of interferon gamma (IFNÞ) decreased in the supernatant of co - cultures
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2015.Ha.I (Browse shelf(Opens below)) Not for loan 01010110069097000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2015.Ha.I (Browse shelf(Opens below)) 69097.CD Not for loan 01020110069097000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Umbilical cord blood (UCB) gained high interest as a source of stem cells for use in cellular therapies. The immune modulatory effect of mesenchymal stem cells (MSCs) originating from bone marrow, adipose tissue and amniotic membrane was previously proved. In this study we aimed at isolation of MSCs from UCB, and evaluate their immune-modulatory effect on peripheral blood lymphocytes proliferation by two different techniques; 5- Bromo - 2- deoxyuridine (Brdu) enzyme linked immunsorbant assay (ELISA) and carboxy Fluorescine diacetate Succinimidyl Ester (CFSE) flow cytometric technique. MSCs were isolated from UCB, propagated until passage 4, characterized for cell surface markers by flow cytometry and ability of mesodermal differentiation was induced towards osteocytes and adipocytes. differentiation was induced towards osteocytes and adipocytes. The immunosuppressive effect on peripheral blood lymphocytes was examined by co - culturing mitomycin C treated UCB MSCs with mitogen-stimulated lymphocytes for 72 hours. Thereafter, proliferation of lymphocytes was detected by CFSE flow cytometry and colorimetric ELISA. The level of cytokines in cell culture supernatant was also assayed to detect the possible mechanism of immunomodulation. UCB MSCs were able to suppress mitogen-stimulated lymphocytes proliferation; this is due to cell-cell contact as well as due to cytokines secretion. The level of transforming growth factor beta (TGFÝ) and interleukin (IL) 10 increased and the level of interferon gamma (IFNÞ) decreased in the supernatant of co - cultures

Issued also as CD

There are no comments on this title.

to post a comment.