The potential gastro protective effect of certain natural products in experimentally induced ulceration in rats / Abeer Mokhtar Bishr ; Supervised Sanaa Abdelbaky Kenawy , Mohammed Mohey ElMazar , Marwa Mohammed Safar

Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology

Peptic ulcer is the most prevalent gastrointestinal disorder. The main cause of gastric injury is the persistent imbalance between defensive and aggressive factors. Many herbal drugs possess gastro protective activity possibly due to their anti-inflammatory and antioxidant effects. This study was conducted to investigate the gastro protective effects of aloe vera, mastic and marshmallow root extracts in rats. Peptic ulcer was induced by indomethacin (30 mg/kg; p.o) & pyloric ligation. Rats were randomly assigned to 6 groups: normal group, gastric ulcer control group; as well as aloe vera (400mg/kg), mastic (1000 mg/kg), marshmallow (200 mg/kg), and ranitidine (150 mg/kg) treated groups. Drugs were orally administered 1 h before ulcer induction. At the end of the experiment animals were sacrificed, gastric fluid was collected and stomachs were isolated and used for estimation of oxidative stress and inflammatory biomarkers as well as histopathological examination Indomethacin-induced gastric ulcer as verified by increment of gastric juice acidity and depiction of focal ulceration in the mucosal lining epithelium associated with hemorrhages in both mucosa and sub mucosal layers in the histological examination accompanied by elevation in gastric oxidative stress biomarkers and inflammatory mediators. All drugs markedly reduced ulcer index, the inflammatory mediator tumor necrosis factor Ü, the apoptotic factor Caspase3, the lipid peroxidation indicator malondialdehyde and leukocyte infiltration marker myeloperoxidase. In addition, the drugs restored the contents of glutathione and the anti-inflammatory cytokine IL-10. Aloe vera and mastic showed significant improvement in histological examination comparable to that exerted by the reference standard ranitidine. These results suggest that aloe vera, mastic and marshmallow may ameliorate peptic ulcer

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