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Effect of mesenchymal stem cells on cisplatin induced nephrotoxicity in albino rats possible role of melatonin / Engy Medhat Ahmed Abdelmegeed ; Supervised Mohamed Abdelaziz Wassef , Salwa Fayez Hassan , Soheir Mahfouz

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Engy Medhat Ahmed Abdelmegeed , 2016Description: 168 P. : charts , facsimiles ; 25cmOther title:
  • تأثير الخلايا الجذعية على التسمم الكلوى المسبب بالسيسبلاتين فى جرذان التجارب البيضاء والدور المحتمل للميلاتونين [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry Summary: Background: Cisplatin is one of the most effective and potent anticancer drugs. It is used in the treatment of a wide variety of both pediatric and adult malignancies. However, the chemotherapeutic use of cisplatin is limited by its serious side effects such as nephrotoxicity. Bone marrow derived mesenchymal stem cells (MSCs) have shown great potential in cell therapy of solid organs. Melatonin and its metabolites possess free-radical scavenging activity and it has been shown that they protect against cisplatin toxicity. So we designed this work to evaluate the effect of MSCs and melatonin on cisplatin induced nephrotoxicity and to study the effect of melatonin on MSCs proliferation. Results: Histopathological examination of kidney tissue from rats which received cisplatin only, revealed the occurrence of nephrotoxicity. Administration of MSCs after induction of experimental nephrotoxicity slightly improved the histopathological picture with residual pathology while administration of melatonin, MSCs together with melatonin and MSCs pretreated with melatonin showed much more improvement of the histopathological picture specially the group of MSCs pretreated with melatonin. Gene expression in rat kidney tissue demonstrated that NFmB and caspase 3 were downregulated in all treated groups with more suppressive effect in the groups treated with MSCs together with melatonin and MSCs pretreated with melatonin specially the pretreated one. Beclin gene expression and Nrf2 protein levels were increased in all treated groups specially in the groups treated with MSCs together with melatonin and MSCs pretreated with melatonin. Serum levels of TNF Ü and MDA were decreased and those of IL-10 and GSH were increased in all treated groups specially the groups treated with MSCs together with melatonin and MSCs pretreated with melatonin. The melatonin treated MSCs showed significant increase in the proliferation rate and BCL2 gene expression and a significant decrease in BAX gene expression
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.03.Ph.D.2016.En.E (Browse shelf(Opens below)) Not for loan 01010110069738000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.03.Ph.D.2016.En.E (Browse shelf(Opens below)) 69738.CD Not for loan 01020110069738000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry

Background: Cisplatin is one of the most effective and potent anticancer drugs. It is used in the treatment of a wide variety of both pediatric and adult malignancies. However, the chemotherapeutic use of cisplatin is limited by its serious side effects such as nephrotoxicity. Bone marrow derived mesenchymal stem cells (MSCs) have shown great potential in cell therapy of solid organs. Melatonin and its metabolites possess free-radical scavenging activity and it has been shown that they protect against cisplatin toxicity. So we designed this work to evaluate the effect of MSCs and melatonin on cisplatin induced nephrotoxicity and to study the effect of melatonin on MSCs proliferation. Results: Histopathological examination of kidney tissue from rats which received cisplatin only, revealed the occurrence of nephrotoxicity. Administration of MSCs after induction of experimental nephrotoxicity slightly improved the histopathological picture with residual pathology while administration of melatonin, MSCs together with melatonin and MSCs pretreated with melatonin showed much more improvement of the histopathological picture specially the group of MSCs pretreated with melatonin. Gene expression in rat kidney tissue demonstrated that NFmB and caspase 3 were downregulated in all treated groups with more suppressive effect in the groups treated with MSCs together with melatonin and MSCs pretreated with melatonin specially the pretreated one. Beclin gene expression and Nrf2 protein levels were increased in all treated groups specially in the groups treated with MSCs together with melatonin and MSCs pretreated with melatonin. Serum levels of TNF Ü and MDA were decreased and those of IL-10 and GSH were increased in all treated groups specially the groups treated with MSCs together with melatonin and MSCs pretreated with melatonin. The melatonin treated MSCs showed significant increase in the proliferation rate and BCL2 gene expression and a significant decrease in BAX gene expression

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