Molecular studies on ganadotrophins in buffalo / Eman Mohamed Abdelfattah Abdelhameed ; Supervised Said Zaki Moustafa Moussa , Adel M. Abou Elfetouh Elbehairy , Eman Moawad Mohamed Gouda

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Biochemistry and Chemistry of Nutrition

The full length of cDNAs encoding FSHÝ, LHÝ and common Ü subunits of gonadotropins of the Egyptian Buffalo were characterized and their sequences were deposited in the GenBank as first record for Egyptian Buffalo. The alignment analysis showed great similarities to Indian buffalo and other ruminants and lower similarities to equines, rodents and primates. Polymorphism in specific loci in beta subunit gene of both FSH and LH was detected using the PCR-SSCP technique followed by sequencing of obtained variants. Three genotypes were found for FSHÝ-2 locus, four genotypes for FSHÝ-3 locus and seven genotypes for LHÝ-2-3 locus. Six out of seven SNPs found in the exon three of FSH were non-synonymous while the SNPs in the other FSH locus and the LHÝ-2-3 locus were synonymous. Significant delay in the age of first conception and first calving was found in FF genotype of LHÝ-2-3 locus in comparison with FG genotype and significant increase in the calving interval was detected in FF genotype when compared to the CC genotype. Significant decrease in both semen volume and 3 hours post thawing motility was recorded in BB genotype of FSHÝ-2 locus when compared to AA genotype with significant decrease in the total sperm abnormalities percent in BB than AA genotype. The CD genotype of FSHÝ-3 locus recorded significant increase in sperm concentration/ml than BC genotype but the 3 hours post thawing motility for CD was significantly lower than both AA and BC genotypes

Issued also as CD