Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy- Department of Pharmacology and Toxicology

The present study was designed to investigate the possible effects of silymarin and grape seed extract (GSE) (100 and 200 mg/kg) either alone or in combination on thioacetamide (TAA)- induced hepatotoxicity in rats with focus on liver function tests and oxidative stress markers. Mature male rats were divided into 7 equal groups (8 rats/group) and treated as follows: Group 1, kept as control group and orally given 0.5 ml 2% tween 80 in distilled water daily, and intraperitoneally (i.p.) injected with sterile distilled water twice weekly for 6 weeks; groups (2-7) were injected with TAA (100 mg/Kg, i.p.) twice weekly for 6 weeks to induce hepatotoxicity. Group 2, orally administered 0.5 ml 2% tween 80 in distilled water daily; groups (3-7) were treated daily with the following drugs two hours prior to TAA injection for 6 weeks to evaluate their hepatoprotective potentials; groups (3-5) were administered daily oral doses of silymarin (50 mg/kg), GSE (100 mg/kg) or GSE (200 mg/kg), respectively. While groups (6-7) were administered combined treatments of silymarin (50 mg/kg) + GSE (100 mg/kg) or GSE (200 mg/kg), respectively. Blood and tissue samples were collected for different biochemical and histological studies

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