Effect of 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG- CoA) Reductase Inhibitor on Experimental Cryptosporidiosis in Immunosuppressed Mice / Noha Madbouly Taha Madbouly ; Supervised Azza Ibrahim Hassan Younis , Hebat Allah Salah Ahmad Yousof , Shaimaa Helmy Elsaid

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Parasitology

The present study was conducted on 200 male mice breeded in Theodore Bilharz Research Institute for the detection of the effect of Atorvastatin on Cryptosporidium parvum infection versus the commercially used drug Nitazoxanide in experimentally immunosuppressed mice. The drug was used in different doses 20mg/kg & 40mg/kg, and each dose was also tested combined with Nitazoxanide. Parasitological and histopathological assessments of the drug effect were done. Parasitological assessment was done using Modified Ziehl Neelsen staining of stool samples collected from mice. Results revealed reduction of the number of oocysts shedded with percentage of reduction in the 21st day post infection of 53.7%, 67.2%, 70.1% & 77.5% respectively compared to infected non-treated group and Nitazoxanide treated group showed percentage of reduction 52.7%. In addition, examination of scrapped small and large intestinal contents after mice scarification revealed reduced numbers of the oocysts shedded with percentage of reduction (56.2%-58.8%, 65.1%-65.3%, 70.6%-73.9% & 77.8%-79.9%) respectively compared to (51.2%- 54.1%) in Nitazoxanide treated group. The histopathological examination of sections from duodenum, jejunum, ileum, colon, stomach & lungs also revealed significant improvement in the Atorvastatin treated groups and remarkable significant improvement in the groups treated with combined drugs

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