Role of resveratrol in ameliorating the biochemical changes in an experimental model of demyelinating disease in mice / Heba Ramadan Ghaiad ; Supervised Amira A. Shaheen , Maha M. Elsawalhi , Mohammed M. Nooh

Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry

Multiple sclerosis (MS) is a demyelinating neurodegenerative disease, representing a major cause of neurological disability in young adults. Resveratrol is a stilbenoid polyphenol, known to pass blood brain barrier and exhibit antioxidant, anti-inflammatory and neuroprotective effects in several brain injuries. Cuprizone model of MS is particularly beneficial in studying demyelination/remyelination. Our study examined the potential neuroprotective and pro-remyelinative effects of resveratrol in cuprizone-intoxicated C57Bl / 6 mice. Mice were fed with chow containing 0.7% cuprizone for 7 days, followed by three weeks on 0.2% cuprizone diet. Resveratrol (250 mg / kg / day, p.o.) was given for 3 weeks starting from the second week. In addition to two normal groups, one untreated and the second treated with resveratrol in similar manner as previously mentioned, to serve as control to the above groups. At the end of the experiment, animals were tested on rotarod to evaluate changes in balance and motor coordination. Mice were then sacrificed to measure the brain content of glutathione, lipid peroxidation products, adenosine triphosphate and phospho- inhibitory subunit of nuclear factor mB-Ü. The activities of cytochrome oxidase and superoxide dismutase were also assessed

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