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Genetic merits of fertility in Egyptian goats / Samira Hamed Sayed Ahmed ; Supervised Said Zaki Mostafa , Eman Moawad Gouda

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Samira Hamed Sayed Ahmed , 2016Description: 163 P. : charts , facsimiles ; 25cmOther title:
  • المميزات الجينية للخصوبة فى الماعز المصرى [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Biochemistry and Chemistry of Nutrition Summary: Aiming to estimate the genotypic and allelic frequencies of FSHÝ and FSHR genes in four Egyptian goat breeds in relation to fertility traits, DNA was isolated from whole blood of 160 animals of Baladi, Parqi, Damascus and Zaraibi goat breeds. PCR-SSCP and sequencing for FSHÝ Exon1 revealed five genotypes and several SNPs with significant positive effect of AA genotype on litter size. Two genotypes and one SNP in restriction site of the enzyme at Intron2, Exon3 locus of FSHÝ gene using PCR- RFLP technique were obtained. Polymorphism using PCR- SSCP of FSHR 5{u2019} regulatory region revealed three genotypes and two SNPs, with AA and BB genotypes had greater litter size in Baladi and Parqi does respectively. While, exon10 of FSHR gene had only one genotype indicating homozygosity of this locus among studied breeds. These results revealed that FSHÝ and FSHR genes could be potential candidate genetic markers for improving goat breeding
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.04.Ph.D.2016.Sa.G (Browse shelf(Opens below)) Not for loan 01010110071181000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.04.Ph.D.2016.Sa.G (Browse shelf(Opens below)) 71181.CD Not for loan 01020110071181000

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Biochemistry and Chemistry of Nutrition

Aiming to estimate the genotypic and allelic frequencies of FSHÝ and FSHR genes in four Egyptian goat breeds in relation to fertility traits, DNA was isolated from whole blood of 160 animals of Baladi, Parqi, Damascus and Zaraibi goat breeds. PCR-SSCP and sequencing for FSHÝ Exon1 revealed five genotypes and several SNPs with significant positive effect of AA genotype on litter size. Two genotypes and one SNP in restriction site of the enzyme at Intron2, Exon3 locus of FSHÝ gene using PCR- RFLP technique were obtained. Polymorphism using PCR- SSCP of FSHR 5{u2019} regulatory region revealed three genotypes and two SNPs, with AA and BB genotypes had greater litter size in Baladi and Parqi does respectively. While, exon10 of FSHR gene had only one genotype indicating homozygosity of this locus among studied breeds. These results revealed that FSHÝ and FSHR genes could be potential candidate genetic markers for improving goat breeding

Issued also as CD

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