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Genotypic variance analysis of Cryptosporidium isolates from stool of children in Egypt / Marwa Mohammed Ibrahim Ghallab ; Supervised Ayman Abdelmoamen Elbadry , Mona Mostafa Khater , Kevin Tyler

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Marwa Mohammed Ibrahim Ghallab , 2016Description: 164 P. : charts , facsimiles ; 25cmOther title:
  • تحليل المتغيرالجيني لطفيل الكريبتوسبوريديوم (البيوغيات الخفية) في عينات براز الأطفال المصريين [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Parasitology Summary: Background: Cryptosporidiosis is recognized by WHO as one of child infectious killers and the second cause of diarhoeal diseases and death in infant. Assessing Cryptosporidium genetic diversity is a realistic goal to elucidate its transmission dynamics and design preventive measures in absence of effective treatment.Objectives: A cross sectional study, was designed to determine the genotypic and sub-genotypic prevalence of Cryptosporidium in stool of Egyptian children using 4 diagnostic methods; AF stain coproscopy, copro- nPCR/RFLP, HRM and sequence analysis of Hsp90, Cowp, Gp60. genes A case control study design was implemented to detect the associated risk factors for susceptibility to cryptosporidiosis. Methodology: Stool samples were collected from 335 children complaining of diarrhea and other GIT symptoms (attending outpatient clinic in Abu El Rish hospital, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt). Their relative data were recorded. Samples were processed for AF stain coproscopy, copro-nPCR/RFLP, HRM and sequence analysis of Hsp90, Cowp, Gp60 genes. Results: Two genotypes C.hominis and C.parvum were identified in 43 isolates from Egyptian children by DNA sequence analysis of COWP and HSP90 genes and by real time PCR high-resolution melting (HRM) assay. Further sub-genotyping phylogenic analysis of DNA sequences at GP60 Abstract locus using PCR-based sequencing identified three C.hominis subtype families (Ib, Id & Ie) and two C. parvum subtype families (IIc & IId)
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.26.Ph.D.2016.Ma.G (Browse shelf(Opens below)) Not for loan 01010110071197000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.26.Ph.D.2016.Ma.G (Browse shelf(Opens below)) 71197.CD Not for loan 01020110071197000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Parasitology

Background: Cryptosporidiosis is recognized by WHO as one of child infectious killers and the second cause of diarhoeal diseases and death in infant. Assessing Cryptosporidium genetic diversity is a realistic goal to elucidate its transmission dynamics and design preventive measures in absence of effective treatment.Objectives: A cross sectional study, was designed to determine the genotypic and sub-genotypic prevalence of Cryptosporidium in stool of Egyptian children using 4 diagnostic methods; AF stain coproscopy, copro- nPCR/RFLP, HRM and sequence analysis of Hsp90, Cowp, Gp60. genes A case control study design was implemented to detect the associated risk factors for susceptibility to cryptosporidiosis. Methodology: Stool samples were collected from 335 children complaining of diarrhea and other GIT symptoms (attending outpatient clinic in Abu El Rish hospital, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt). Their relative data were recorded. Samples were processed for AF stain coproscopy, copro-nPCR/RFLP, HRM and sequence analysis of Hsp90, Cowp, Gp60 genes. Results: Two genotypes C.hominis and C.parvum were identified in 43 isolates from Egyptian children by DNA sequence analysis of COWP and HSP90 genes and by real time PCR high-resolution melting (HRM) assay. Further sub-genotyping phylogenic analysis of DNA sequences at GP60 Abstract locus using PCR-based sequencing identified three C.hominis subtype families (Ib, Id & Ie) and two C. parvum subtype families (IIc & IId)

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