Therapeutic effect of holothuria arenicola against hepatorenal injury in septic rats / Maha Yassin Abdalshakour Abdelhady ; Supervised Sohair Ramadan Fahmy , Amel Mahmoud Ali Soliman , Amany Ahmed Sayed Ali

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

Sepsis is still a major challenge in contemporary medicine. Clinically, massive bacterial contamination is the common cause of sepsis during surgery. The invasion of such pathogens to normal organs causing multiorgan failure including liver and kidney via oxidative stress (OxS). Cecal ligation and puncture (CLP) is a gold standard animal model of sepsis. The aim of the present study was to assess the complications of hepatorenal injury associated with sepsis and to evaluate the therapeutic effect of holothuria arenicola body wall extract (HABWE) and Holothuria arenicola coelomic fluid extract (HACFE) in hepatorenal damage induced by CLP. Eighty-four male rattus norvegicus rats were divided into four groups, the sham-operated control, CLP, HABWE and HACFE treated groups. Both sham and CLP groups administered distilled water, while HABWE and HACFE treated groups administered (200 mg/kg body weight) for 3 days after CLP surgery. The results revealed that, CLP operation induced OxS in rats, which have bad impacts on liver and kidney functions confirmed by the histopathological examination. Also, sepsis changed the hematological parameters, procalcitonin and cytochrome P 1A2 levels than the normal control values. However, HABWE and HACFE showed their ability to reduce OxS, which leads to significant improvement in most of the biochemical parameters. Also, histopathological examination showed an improvement in the liver and the kidney architectures of the treated groups as compared to CLP group

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