Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry

Azadirachtaindica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. Our study aims to investigate in vivo antitumor, and antioxidant activities of ethanolic neem leaf extract (ENLE) and its fraction nimbolide, a limonoid present in leaves and flowers of the neem tree (Azadirachtaindica). Also, to study the side effects of ENLE and nimbolide fraction on different organs (liver / kidney). Our results showed that Nimbolide and ENLE showed great increase in life span. Also, they showed a significant decrease in malondialdehyde and nitric oxide and an increase in catalase, glutathione peroxidase, caspase-3 activities, and cytochrome c concentration. Hence, it may be possible that Nimbolide and ENLE decrease lipid peroxidation level due to their antioxidant effect and enhance apoptosis process. ENLE and nimbolide showed no side effects on liver and kidney. Also, they showed a significant protection for both liver and kidney histopathologically

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