Detection of schistosoma haematobium dna in urine of patients with cancer bladder in Egypt / Shimaa Hussien Ali ; Supervised Safeya Omar Elbassiouni , Hala M. Samir Elaskary , Dina Hamdy Elakkad

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Parasitology

Schistosomes are long-lived parasites, hence schistosomiasis is a chronic disease with severe long-term implications. However, definitive diagnosis of active infection has been difficult because demonstration of infection has depended on detecting parasite eggs in urine and/or stool. In the case of Schistosoma haematobium which parasitizes the urogenital system, this method has low sensitivity in adults. Parasite-specific DNA in urine of patients with schistosomiasis has been previously detected .The aim of the current study was the detection of Schistosoma haematobium DNA in urine of patients with cancer bladder in Egypt and investigate schistosoma specific DNA role in diagnosis of infection in patients with chronic Schistosoma infection. This study was conducted on sixty patients with cancer bladder. Cases included in the present work were subjected to parasitological examination of urine sediment after centrifugation for the detection of S.haematobium eggs and for investigation of S.haematobium DNA in urine samples. By parasitological examination no eggs of S.haematobium were detected in all 60 patients. By multiplex PCR it was revealed that 5 cases (8.3%) were positive for S.haematobium, while 55 cases (91.7%) were negative. A statistically significant positive relationship between PCR results and cystoscopic biopsy reports P-value equal 0.003 R=0.378 has been demonstrated

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