Serological and molecular studies on Theileria equi in Egyptian equine / Bassma Said Mohamed Elsawy ; Supervised Olfat Anter Mahdy , Ahmed M. Nassar , Mona Said Mahmoud
نوع المادة :
نصاللغة: الإنجليزية تفاصيل النشر: Cairo : Bassma Said Mohamed Elsawy , 2016 الوصف: 85 P. : charts , facsimiles ; 25cmعنوان آخر: - دراسات سيرولوجية و جزيئية علي ثيليريا إكواي في الخيول المصرية [عنوان مضاف عنوان الصفحة]
- Issued also as CD
| نوع المادة | المكتبة الحالية | المكتبة الرئيسية | رقم الاستدعاء | رقم النسخة | حالة | الباركود | |
|---|---|---|---|---|---|---|---|
Thesis
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قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.10.12.M.Sc.2016.Ba.S (استعراض الرف(يفتح أدناه)) | لا تعار | 01010110070976000 | ||
CD - Rom
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مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.10.12.M.Sc.2016.Ba.S (استعراض الرف(يفتح أدناه)) | 70976.CD | لا تعار | 01020110070976000 |
استعرض المكتبة المركزبة الجديدة - جامعة القاهرة رفاً إغلاق مستعرض الرف (يخفي مستعرض الرف)
| لا توجد صورة غلاف متاحة | لا توجد صورة غلاف متاحة | لا توجد صورة غلاف متاحة | لا توجد صورة غلاف متاحة | لا توجد صورة غلاف متاحة | لا توجد صورة غلاف متاحة | لا توجد صورة غلاف متاحة | ||
| Cai01.10.12.M.Sc.2014.So.S Studies on some metacestodes infecting ruminants / | Cai01.10.12.M.Sc.2016.As.S Some studies about theileria spp. among sheep in Egypt / | Cai01.10.12.M.Sc.2016.As.S Some studies about theileria spp. among sheep in Egypt / | Cai01.10.12.M.Sc.2016.Ba.S Serological and molecular studies on Theileria equi in Egyptian equine / | Cai01.10.12.M.Sc.2016.Ba.S Serological and molecular studies on Theileria equi in Egyptian equine / | Cai01.10.12.M.Sc.2016.Ho.S Some studies on the control of sheep blowfly lucilia sericata (Diptera: Calliphoridae) in Egypt / | Cai01.10.12.M.Sc.2016.Ho.S Some studies on the control of sheep blowfly lucilia sericata (Diptera: Calliphoridae) in Egypt / |
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Parasitology
Equine piroplasmosis (EP) is a disease of equids which caused by the blood-borne protozoan parasite Theileria (Babesia) equi. The incidence of Theileria equi infection was studied in 301 equine samples (133 donkeys and 168 horses) from Giza and Cairo governorate using microscopic examination (ME), competitive ELISA (cELISA), indirect ELISA (iELISA) and nested (nPCR). The used antigen in iELISA was prepared from blood of naturally infected splenectomized donkey at the peak of parasitemia. In ME, the parasite was detected in 79 (26.2%) equine blood samples; 33 donkeys and 46 horses with an incidence rate (24.8% and 27.4%), respectively. The T. equi antibodies were detected with cELISA in 60 (19.9%) equine serum samples, where 34 donkeys and 26 horses with an incidence rate (25.6% and 15.5%), respectively. The incidence rate in equine samples using iELISA was (33.5%) from which 71 donkeys and 30 horses were infected (53.4% and 17.9%), respectively. The nPCR based on the T. equi merozoite antigen gene (EMA-1) allowed the visualization of species-specific amplified product in 171 (56.8%) equine blood samples, 67 donkeys and 104 horses with an incidence rate (50.4% and 61.9%), respectively. Approximately 229 bp of the ema-1 gene from 3 Egyptian samples were sequenced and BLASTN analysis confirmed all sequences to be merozoite surface protein genes, with an identity of 100% to previously published Babesia equi merozoite antigen- 1 (EMA-1) gene reference sequence (our GenBank Accession number KX262963). Statistical analysis using Chi square indicated significant differences (P< 0.05) between ME and nPCR; ME and iELISA; nPCR and cELISA and nPCR and iELISA in total equine on the detection of parasite carriers. In conclusion, the most sensitive technique in diagnosis of T. equi infection is nPCR, followed by cELIZA, iELISA and ME. The combination of ELISA and PCR was recommended for detection of acute and chronic stage
Issued also as CD
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