Pathological studies on hepatic tissues of slaughtered cattle and buffaloes / Islam Elgohary Ahmed Elgohary ; Supervised Mahmoud Aly Mahmoud , Reham Mahmoud Mohamed , Nasser Gamal Eldine

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Clinical Pathology

The liver is the most important organ in the body, any hepatic affection leading to decrease animal production and reproduction causing high economic losses, on other hand the liver is edible part in Egypt. So any affection on it will affect human health. In our study, during August 2014 to August 2015, two hundreds and thirteen liver samples of slaughtered cattle and buffaloes were collected from Elbastin abattoir, Cairo governorate, Egypt for parasitological, bacteriological , toxicological and pathological examination. The overall incidence of fasciola infestation was 7.3% while, the bacterial infection was 0.5% for S,aureus and 0.5% for C.Pseudotuberculosis. The incidence rate of aflatoxicosis in representative samples (39 samples) using TLC was 79.5% (where 31 samples out of 39 were positive). Using LC MS/MS, nine samples were examined and found to be affected by AFM1. Histopathology in case of fasciolasis showing biliary hyperplasia and portal fibrosis with infiltration by esinophiles , neutrophiles and mononuclear cells. In case of S.aureus, presence of caseous necrosis surrounded by massive number of neutrophiles and fibrous connective tissue capsule were characteristic while presesnce of liquifactive necrosis surrounded by neutrophiles were common in case of C.pseudotuberculosis. the samples affected by AFs showing endophelbitis in portal veins and portal fibrosis. Hyperplasia in bile ductule was also seen together with congestion in hepatic sinusoid and portal blood vessel. On the other hand comet test showing gentoxic effect of aflatoxin on DNA of the examined hepatocytes

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