Detection of antibiotic resistant propionibacterium acnes isolated from patients with acne vulgaris / Rasha Anter Metwally Elfekki ; Supervised Nadia Hafez Salah Eldin Ouda , Nesrine Samir Abdelfatah , Manal Mohammed Sulaiman Hallal

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

Propionibacterium acnes (P. acnes) has been implicated in the pathogenesis of acne since the beginning of the last century. Over several decades, topical and systemic antibiotics have been the main line of treatment for acne vulgaris. However, in the present era of increased antibiotic usage, resistant strains have emerged. The aim of this study is to determine the antibiotic resistance pattern among P. acnes isolated from patients with acne vulgaris at the Dermatology clinics of Kasr Alainy teaching hospital. Specimens were extracted from the pustules and taken by sterile cotton swabs and transported by thioglycolate media. Each swab was inoculated onto two blood agar plates, one incubated aerobically at 37{u00BA}C for 24h and the other anaerobically for one week. P. acnes was identified by Gram stain and biochemical tests. Their susceptibility pattern to doxycyclin, erythromycin, clindamycin, tetracycline, azithromycin and trimethoprim-sulfamethoxazole was determined on Muller Hinton media by disc diffusion method. A total of 44 P. acnes isolates were identified from 100 patients with acne vulgaris, out of which 22.7% were resistant to clindamycin, 11.4% were resistant to trimethoprim-sulfamethoxazole and 9% were resistant to erythromycin. Resistance to doxycycline, tetracycline or azithromycin was not detected. Trimethoprim-sulfamethoxazole showed statistically significant difference in the resistance pattern compared to patient{u2019}s sex (p=0.029) and to receiving previous treatment p=0.018

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