Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Histology and Cytology

The liver of twenty adult apparently healthy water buffaloes of both sexes investigated anatomically, histologically, histochemically, electron microscopy, immunohistochemically and immunofluorescently, In addition, the normal levels of glutathione and trace minerals were estimated. Thick covering capsule and well developed connective tissue in the portal area were observed. Moreover reticular fibers were detected in different manners; network and dispersed form. Solitary bile ducts were distributed along the hepatic parenchyma. Ultrastructuraly; oval cells with electro-lucent nucleus and scanty cytoplasm were demonstrated among the cells lining the bile duct. Immunohistochemically, von Kupffer cells revealed positive reaction to CD68 and Ito cells gave more intense reaction to Anti-vimentin than Anti-desmin. On the other hand, the latter showed high reactivity for vitamin A immunofluorescence. The most obvious result in this study was the insulin and glucagon immunoreactivity. The mean values of trace minerals were higher in iron, copper and zinc.

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