Studies on propagation of some peach rootstocks during tissue culture technique / Esraa Alaa Eldin Kassem Said Fayed ; Supervised Gamal Mohamed Mahmoud Haseeb , Samy Elkosary Melegy , Elsayed Ibrahim Bakr

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Esraa Alaa Eldin Kassem Said Fayed

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TextLanguage: English Publication details: Cairo : Esraa Alaa Eldin Kassem Said Fayed , 2016Description: 99 P. : facsimiles ; 25cmOther title:

درا{uئإآ٣}{uئإ٨إ}ت {uئإأآ}{uئإإ٠}{uئإئ٠} أ{uئإؤآ}{uئإ٩أ}{uئإ٨إ}ر {uئإ٩١}{uئإأأ}{uئإآإ} ا{uئإآآ}{uئإإإ}ل ا{uئإؤئ}{uئإء٨}{uئإإإ}خ {uئإ٩١}{uئإ٨إ}{uئإآ٣}{uئإ٩٨}{uئإء٨}{uئإءء}ام {uئإ٩٧}{uئإؤ٨}{uئإإ٨}{uئآئئ}{uئإ٩٤} زرا{uئإأآ}{uئإ٩٤} ا{uئإئآ}{uئإإ٧}{uئإآ٤}{uئإء٠}{uئإ٩٤} [Added title page title]

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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Pomology Summary: This research aimed to facilitate detection and selection of salt stress tolerance of peach rootstocks. Also, to create a reliable protocol for callus induction and regeneration of peach [prunus persica (L.) batsch] rootstocks; montclair, nemaguard and okinawa, using mature seeds as in vitro establishment plant materials where each seed cultured separately and tagged with an unique serial number. Seeds recorded the maximum germination% when disinfested using 20% clorox for 30 minutes prior to culture on woody plant medium as compared to murashige and Skoog medium for all tested rootstocks. The combination of 1.5 mg/l BA+0.2 mg/l GA3 produced sufficient number of shoots with suitable height for all tested rootstocks during multiplication stage. After establishment stage, all the three tested rootstocks succeed in callus formation with maximum mass by adding 1.0 mg/l IBA+1.0 mg/l NAA to half-strength MS (Murashige and Skoog) culture medium in the presence of 1 g\l PVA (polyvinyl alcohol). Callus clusters of montclair rootstock, which recorded the highest callus induction frequency on different auxin treatments, were differentiated when transferred to free PVA and growth regulators MS medium. The highest concentration of NAA (2.0 mg/l) was more effective regarding root formation specially when added combining with the highest IBA concentration 2.0 mg/l. Inter Simple Sequence Repeat technique could confirmed the role of fragments polymorphism of Montclair rootstock differentiated plantlets identification and discrimination as compared to directly regenerated one from the origin individual. Root tip cultures, 1 cm in length, were evaluated for salt stress tolerance after one month using liquid half-strength MS medium containing 0 (control), 400, 800, 1200, 1600 or 2000 ppm NaCl this stage was made after establishment

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Holdings
Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.07.13.M.Sc.2016.Es.S (Browse shelf(Opens below))		Not for loan		01010110072116000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.07.13.M.Sc.2016.Es.S (Browse shelf(Opens below))	72116.CD	Not for loan		01020110072116000

Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Pomology

This research aimed to facilitate detection and selection of salt stress tolerance of peach rootstocks. Also, to create a reliable protocol for callus induction and regeneration of peach [prunus persica (L.) batsch] rootstocks; montclair, nemaguard and okinawa, using mature seeds as in vitro establishment plant materials where each seed cultured separately and tagged with an unique serial number. Seeds recorded the maximum germination% when disinfested using 20% clorox for 30 minutes prior to culture on woody plant medium as compared to murashige and Skoog medium for all tested rootstocks. The combination of 1.5 mg/l BA+0.2 mg/l GA3 produced sufficient number of shoots with suitable height for all tested rootstocks during multiplication stage. After establishment stage, all the three tested rootstocks succeed in callus formation with maximum mass by adding 1.0 mg/l IBA+1.0 mg/l NAA to half-strength MS (Murashige and Skoog) culture medium in the presence of 1 g\l PVA (polyvinyl alcohol). Callus clusters of montclair rootstock, which recorded the highest callus induction frequency on different auxin treatments, were differentiated when transferred to free PVA and growth regulators MS medium. The highest concentration of NAA (2.0 mg/l) was more effective regarding root formation specially when added combining with the highest IBA concentration 2.0 mg/l. Inter Simple Sequence Repeat technique could confirmed the role of fragments polymorphism of Montclair rootstock differentiated plantlets identification and discrimination as compared to directly regenerated one from the origin individual. Root tip cultures, 1 cm in length, were evaluated for salt stress tolerance after one month using liquid half-strength MS medium containing 0 (control), 400, 800, 1200, 1600 or 2000 ppm NaCl this stage was made after establishment

Issued also as CD

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