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Studies on the effect of gold nanoparticles on diagnostic polymerase chain reaction PCR technique / Dalia Mostafa Elhusseini Mohamed ; Supervised Reham Helmy Tammam , Nashwa Mohamed Helmy

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Dalia Mostafa Elhusseini Mohamed , 2017Description: 115 P. : charts , photographs ; 25cmOther title:
  • دراسات على تأثير جسيمات نانو الذهب على تقنية تفاعل إنزيم البلمرة المتسلسل التشخيصى [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology Summary: Polymerase chain reaction (PCR) technique is considered to be one of the most highlighted and promising applications in molecular diagnostics. Conventional PCR could results in false negatives due to low nucleic acid copy number in low titer samples which rises the necessity to improve the technique{u2019}s efficiency. In this study, we used equine Herpes Virus-1 (EHV-1) DNA as a template to determine the effect of 15 nm unmodified gold nanoparticles (GNPs) on the key PCR reactants. The overall reaction outcomes has been enhanced after the optimization of the reaction regarding the GNPs, primers and taq polymerase concentrations for more efficient, highly sensitive molecular detection of EHV-1. The optimized GNPs assisted PCR resulted in specific high yield amplification with a detection limit of 100 DNA copies. The sensitivity of the optimized technique was 92.3% in comparison to 68.6% of conventional PCR which permit the EHV-1 detection in ~11% more samples than conventional PCR
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2017.Da.S (Browse shelf(Opens below)) Not for loan 01010110072870000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2017.Da.S (Browse shelf(Opens below)) 72870.CD Not for loan 01020110072870000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology

Polymerase chain reaction (PCR) technique is considered to be one of the most highlighted and promising applications in molecular diagnostics. Conventional PCR could results in false negatives due to low nucleic acid copy number in low titer samples which rises the necessity to improve the technique{u2019}s efficiency. In this study, we used equine Herpes Virus-1 (EHV-1) DNA as a template to determine the effect of 15 nm unmodified gold nanoparticles (GNPs) on the key PCR reactants. The overall reaction outcomes has been enhanced after the optimization of the reaction regarding the GNPs, primers and taq polymerase concentrations for more efficient, highly sensitive molecular detection of EHV-1. The optimized GNPs assisted PCR resulted in specific high yield amplification with a detection limit of 100 DNA copies. The sensitivity of the optimized technique was 92.3% in comparison to 68.6% of conventional PCR which permit the EHV-1 detection in ~11% more samples than conventional PCR

Issued also as CD

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