Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Endodontics

The present study aimed to evaluate the effect of Gamma radiation on the biocompatibility and surface texture of MTA, Ketac-Fil and Ketac-silver. Baby hamster kidney fibroblast cell line was used in the study. The samples were divided into three main groups according to the materials used. Each group was classified into two sub groups that were tested before and after radiations. The latter group was subject to fractional gamma radiations. According to the dose which was used in radiotherapy which vary from 50-70 Gy, a total dose of 60 gray was delivered in fractions of 20 Gy/each session (20/1). The test materials were gamma irradiated in Gamma chamber installed in National Centre for Radiation Research and Technology (NCCRT). Slides were photomicrographed at the power of 1000x oil immersion. This was done using a digital video camera. Baby hamster kidney fibroblasts (BHKF) normally appear as bipolar or multipolar cells, have elongated shapes, and grow attached to a substrate. The cells from the culture plate are considered morphologic markers of cytotoxicity. Therefore, images of BHKF treated with different concentrations of MTA were examined by using phase contrast microscopy. Irrespective of material, radiation and different concentrations, it was found that one-day incubation time recorded a statistically significant (p < 0.05) highest cell viability % (i.e. less cytotoxic) followed by three days' incubation time while seven days' incubation recorded statistically significant (p < 0.05) lowest cell viability % (i.e. more cytotoxic). Pair-wise Tukey{u2019}s post-hoc test showed non-significant (p>0.05) differences between (three days and seven days). Regardless of irradiation, incubation time and different concentrations, it was found that MTA group recorded statistically significant (p < 0.05) highest cell viability % followed by Ketac-Silver group while the lowest statistically significant cell viability % was recorded by Ketac-Fil group. Tukey{u2019}s pair-wise post-hoc test showed non-significant difference (p>0.05) between control group and MTA group. Ketac-Fil and Ketac-Silver groups showed statistical significance (p<0.05) when compared with control group.

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