Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Analytical Chemistry

This thesis described simultaneous analysis of binary or ternary mixturesof some drugs used in the treatment of GIT disorders by applying spectrophotometric, chemometric-assisted spectrophotometric and chromatographic methods.The aim of work was to develop several simple, accurate, sensitive, selective and precise methods for the assay of the cited drugs (mebeverine hydrochloride, chlordiazepoxide and clidinium bromide) in their pure powdered form, laboratory-prepared mixtures and pharmaceutical formulation. The first chapter included a general introduction about the chemistry, mode of action and literature review of the reported analytical methods of mebeverine hydrochloride (MVH) and chlordiazepoxide (CDZ). Different manipulating pathways were applied for simultaneous determination of mixtures of MVH and CDZ in their pure powdered form and pharmaceutical formulation using either successive or progressive resolution techniques without prior separation steps. These pathways were applied either on zero-order absorption spectra namely, absorbance subtraction (AS) or based on the recovered zero-order absorption spectra via a decoding technique namely, derivative transformation (DT) or on ratio spectra namely, ratio subtraction (RS) coupled with extended ratio subtraction (RS-EXRS), spectrum subtraction (RS-SS), constant multiplication (RS-CM) and constant value (CV) methods. The manipulation steps applied on the ratio spectraof zero-order absorption spectra are namely, ratio difference (RD) and amplitude modulation (AM) methods or applying a derivative to these ratio spectra namely, derivative ratio (DD1) or second derivative (D2). Finally, the pathway based on the ratio spectra of derivative spectra is namely, derivative subtraction (DS). The specificity of the developed methods was investigated by analyzing laboratory-prepared mixtures and pharmaceutical formulation containing the cited drugs with no interference from additives. The proposed methods were validated according to ICH guidelinesnce between them for the determination of MVH and CDZ regarding accuracy and precision

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