Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

The contamination of cell cultures by Mycoplasma remains a major problem in cell culture and vaccine manufacture. Mycoplasma can produce a virtually unlimited variety of effects in the cultures they infect. These organisms are resistant to most antibiotics commonly employed in cell cultures. Here we provide a concise overview of the current knowledge on: (1) the incidence and sources of Mycoplasma contamination in cell cultures, the Mycoplasma species most commonly detected in cell cultures, and the effects of Mycoplasma on the function and uses of infected cell cultures (2) the various techniques available for the detection of Mycoplasma with particular emphasis on the most reliable detection methods and (3) Phylogenic analysis of Mycoplasma strains isolated from Local veterinary viral Vaccines. The availability of accurate, sensitive and reliable detection methods and the application of robust and successful elimination methods provide powerful means for overcoming the problem of Mycoplasma contamination in vaccines manufacture

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