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Study on sources and effect of microbial contamination on stability of certain non-sterile preserved oral pharmaceutical products / Lobna Aref Abdelrazak Bahnsi Deif ; Supervised Alaa Eldin Mahmoud Shawky Hosny , Rania Abdelmonem Mostafa Khattab

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Lobna Aref Abdelrazak Bahnsi Deif , 2017Description: 66 P. : charts , facsimiles ; 25cmOther title:
  • دراسة عن مصادر و تأثير الملوثات الميكروبية على ثبات بعض المستحضرات الصيدلية الغير عقيمة المحفوظة و المستخدمة عن طريق الفم [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology Summary: In this study, two types of preserved syrup with different preservative were chosen to study types, sources of contaminants including environmental condition as air, raw materials and water. From 400 different samples of finished product, 3 bacterial and 39 fungal isolates had been isolated. However, the following microbes isolated: 64 fungal isolates in addition to 188 bacterial isolates were isolated from air samples.Only four bacterial isolates were isolated from sucrose. However, microbiological analysis of other raw material did not result in any bacterial or fungal isolates. Finally, only three bacterial isolates were isolated from water included in formulation of finished product. Aspergillusflavuswasthemostcommonfungusisolatedamong 31and25fungalisolates fromair andfinished syrupsamplesrespectively. The genetic relationship between numberof isolated Asp. flavus (20randomsamples) using RAPD PCR technique was studiedandillustratedin form of dendrogram. Obtaineddendrogramshowedthattherewasgeneticrelatednessbetweenfungiisolatedfromairandfungiisolatedfromfinishedproductwith72%to32%
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.08.06.M.Sc.2017.Lo.S (Browse shelf(Opens below)) Not for loan 01010110073902000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.08.06.M.Sc.2017.Lo.S (Browse shelf(Opens below)) 73902.CD Not for loan 01020110073902000

Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

In this study, two types of preserved syrup with different preservative were chosen to study types, sources of contaminants including environmental condition as air, raw materials and water. From 400 different samples of finished product, 3 bacterial and 39 fungal isolates had been isolated. However, the following microbes isolated: 64 fungal isolates in addition to 188 bacterial isolates were isolated from air samples.Only four bacterial isolates were isolated from sucrose. However, microbiological analysis of other raw material did not result in any bacterial or fungal isolates. Finally, only three bacterial isolates were isolated from water included in formulation of finished product. Aspergillusflavuswasthemostcommonfungusisolatedamong 31and25fungalisolates fromair andfinished syrupsamplesrespectively. The genetic relationship between numberof isolated Asp. flavus (20randomsamples) using RAPD PCR technique was studiedandillustratedin form of dendrogram. Obtaineddendrogramshowedthattherewasgeneticrelatednessbetweenfungiisolatedfromairandfungiisolatedfromfinishedproductwith72%to32%

Issued also as CD

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