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Production of Ý- galactosidase Enzyme and it's Industrial Application / Hassanein Abdelaziz Hassanein ; Supervised Olfat Sayed Barakat , Hayam Mohamed Fathy , Gamal Ahmed Mohamed

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hassanein Abdelaziz Hassanein , 2017Description: 108 P. : charts , facsimiles ; 25cmOther title:
  • انتاج انزيم البيتاجلاكتوسيديز و تطبيقاته الصناعية [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology Summary: Twenty strains of lactic acid bacteria (LAB) were isolated from Kareish cheese in Cairo markets. The results indicated that Streptococcus thermophilus and Lactobacillus bulgaricus were the most efficient Ý-gal activity producers with specific activity of 2.50 and 2.09 U/mg protein, respectively. These strains were immobilized by barium alginate 2% entrapped cells. Ý- Galactosidase from These strains was immobilized on barium alginate 2% and activity and stability were evaluated. Lactose free milk produced from Skimmed Milk by immobilized these strains using fed-batch culture. L. bulgaricus 10% showed the most efficiency of 85.26%. Lactose free milk produced by immobilized Ý- Galactosidase using continuous process with four flow rate. The flow rate 0.1ml/min was the most efficient (92.89 %). Saccharification of whey lactose by immobilized Ý-Galactosidase using continuous process with flow rate 0.25 ml/min gave efficiency of (73.05%).in conclusion immobilized bioreactor containing immobilized enzyme is recommended for continuous production of lactose free milk at flow rate 0.1 ml/min
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.06.M.Sc.2017.Ha.P (Browse shelf(Opens below)) Not for loan 01010110074103000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.06.M.Sc.2017.Ha.P (Browse shelf(Opens below)) 74103.CD Not for loan 01020110074103000

Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology

Twenty strains of lactic acid bacteria (LAB) were isolated from Kareish cheese in Cairo markets. The results indicated that Streptococcus thermophilus and Lactobacillus bulgaricus were the most efficient Ý-gal activity producers with specific activity of 2.50 and 2.09 U/mg protein, respectively. These strains were immobilized by barium alginate 2% entrapped cells. Ý- Galactosidase from These strains was immobilized on barium alginate 2% and activity and stability were evaluated. Lactose free milk produced from Skimmed Milk by immobilized these strains using fed-batch culture. L. bulgaricus 10% showed the most efficiency of 85.26%. Lactose free milk produced by immobilized Ý- Galactosidase using continuous process with four flow rate. The flow rate 0.1ml/min was the most efficient (92.89 %). Saccharification of whey lactose by immobilized Ý-Galactosidase using continuous process with flow rate 0.25 ml/min gave efficiency of (73.05%).in conclusion immobilized bioreactor containing immobilized enzyme is recommended for continuous production of lactose free milk at flow rate 0.1 ml/min

Issued also as CD

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