Application of advanced analytical techniques for determination of some pharmaceutical formulations possessing an analgesic effect / Souha Hosam Kamal Youssef ; Supervised Amr Mohamed Badawy , Maha Abdel Monem Hegazy , Dalia Mohamed Mamdouh Abdelwahab

Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Analytical Chemistry

The analgesics market is growing steadily worldwide. Not only do they exist in single dosage forms but also as mixtures in pharmaceutical formulations for synergistic effect. Quantitative analysis of pharmaceuticals is necessary throughout the various stages of drug development and manufacture. It is considered advantageous to develop accurate and rapid methods which are of low cost and do not require hazardous solvents and prolonged sample pretreatment. Two ternary mixtures were analyzed in this study; the first mixture was composed of paracetamol, pseudoephedrine hydrochloride and brompheniramine maleate and the second mixture was composed of paracetamol, pseudoephedrine hydrochloride and cetirizine dihydrochloride. Different analytical techniques were applied for the quantitative determination of these mixtures. The applied techniques included; chromatographic methods (thin layer chromatography, high performance liquid chromatography with UV - detection, ultra-performance liquid chromatography coupled with mass spectroscopy), Spectrophotometric methods (derivative, ratio subtraction coupled with ratio difference, successive derivative ratio, successive derivative subtraction and derivative ratio spectrum - zero crossing methods) and chemometric methods (Principle component regression, partial least square, pure component contribution algorithm and concentration residual augmented classical least squares methods). The developed methods were effectively applied for the analysis of laboratory prepared mixtures as well as the dosage forms. Statistical comparison of the obtained results by the proposed methods and the reported ones has revealed no significant difference regarding accuracy and precision. Validation was conducted according to the ICH guidelines confirming the method to be selective, linear, precise and accurate

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