Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Vegetable

This study was carried out during the period from 2010 to 2014 in the plant tissue culture laboratory and the farm of the PICO comp. at Giza and Elbehaira government, respectively, Egypt. The aim of this work was to study the effect of subculture number and benzyl Adenine (BA) concentration on the production of strawberry plants. Meristem tip of three strawberries (Fragaria x ananassa) cultivars, namely, sweet charlie, festival and camarosa, were cultured on MS basal medium (murashige and skoog, 1962) free-hormone for 4 weeks and subcultured on media having three different concentrations (0.5, 0.25, 0.10 mg/l) of BA. The highest multiplication rate was obtained in the sixth subculture with BA at 0.5 mg for sweet charlie. The performances of tissue culture-derived plants for fruiting under open field conditions, revealed that the highest percentage of marketable yield in the early, medium and late pickings was noticed in all cultivars in the sixth plants with using BA at 0.10 mg/l, Also the greatest early yield (3.041 kg/m2) was recorded for the plants of {u2019}sweet charlie {u2018}produced from the fifth subculture in medium had BA at 0.5. The greatest medium yield (2.705 kg/m2) was recorded for the plants of {u2019}sweet charlie {u2018}produced from the fifth subculture in medium had BA at 0.5 mg, the highest late yield (3.443 kg/m2) was recorded for the plants of {u2019}sweet charlie{u2018}produced from the sixth subculture in medium had BA at 0.25 mg. The heaviest average fruit weight during early pickings (29.66 g) was recorded for the plants of sweet charlie produced from the sixth subculture in medium having BA at 0.25 mg

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