Detection of Schistosoma DNA in biopsies from Egyptian Patients with Urinary Bladder Cancer variants / Shimaa Sayed Ibrahim Dawood ; Supervised Olfat Mohamed Mahmoud Elmatrawy , Hosni Khairy Salem , Samah Sayed Mohammed Abdelgawad
Material type: TextLanguage: English Publication details: Cairo : Shimaa Sayed Ibrahim Dawood , 2017Description: 125 P. : charts , facsimiles ; 25cmOther title:- الكشف عن الحمض النووى للبلھارسيا فى خزعات من مرضى مصريين مصابين بسرطان المثانة البولية المختلفة [Added title page title]
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Item type | Current library | Home library | Call number | Copy number | Status | Date due | Barcode | |
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Thesis | قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.11.26.Ph.D.2017.Sh.D (Browse shelf(Opens below)) | Not for loan | 01010110074324000 | |||
CD - Rom | مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.11.26.Ph.D.2017.Sh.D (Browse shelf(Opens below)) | 74324.CD | Not for loan | 01020110074324000 |
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Parasitology
Background : Schistosomiasis is a chronic disease with severe long-term implications. Definitive diagnosis of active infection is difficult because it depends on detecting parasite eggs in urine and/or stool. This method has low sensitivity in adults. Parasite-specific DNA in patients with schistosomiasis has been previously detected. Objectives: The aim of the current study is to document the association between the burden of urinary bladder cancer variant (SCC/TCC: Squamous Cell Carcinoma/ Transitional Cell Carcinoma) and schistosomiasis using molecular techniques and to correlate the pathological detection of Schistosoma egg granuloma and Schistosoma DNA in bladder tissue of Egyptian patients with urinary bladder cancer. Material and methods: This study was conducted on 100 patients with bladder cancer. Cases included in the present work were subjected to pathological examination of tissue samples for analyzing the type of Cancer/ histopathologically with grading according to the degree of invasiveness and presence or absence of Schistosoma egg granuloma.Also cases will be investigated to detect S. haematobium DNA in their bladder tissue samples. Results: Histopathological data showed the prescence of Schistosoma eggs in 18 case (18%) from all study population and all of them were associated with SCC (18 /40) about 45% from cases with SCC. No eggs were detected in other types of tumors, which is a significant finding as P-Value=<0.0001. Multiplex PCR could reveal the positivity of 21 cases. Among these cases the number of 20 were histopathologically associated with schistosomiasis and of SCC type and one case had bladder cancer of the TCC type but was not associated with schistosomiasis
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