Comparative study between different laboratory methods in the diagnosis of human brucellosis / Sara Elhossain Aly Reda Mohamed ; Supervised Mona Abdelwahab Abdelmessih , Abdelmeguid Kassem , Lamiaa Abdelfattah Ahmed Madkour

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Sara Elhossain Aly Reda Mohamed

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TextLanguage: English Publication details: Cairo : Sara Elhossain Aly Reda Mohamed , 2017Description: 137 P. : charts , facsimiles ; 25cmOther title:

دراسة مقارنة للطرق المعملية المختلفه لتشخيص مرض الحمى المالطيه فى الانسان [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology Summary: The aim of the study was to compare the performance of different serological tests in the diagnosis of brucellosis, considering quantitative real time PCR (qPCR) as the reference test. Quantitative real time PCR was positive for Brucella DNA in 65% out of 100 serum samples. Both the standard tube agglutination test (STAT) and rose bengal (RB) test revealed high sensitivity in the detection of brucellosis (95% and 89% respectively). The positive predictive value (PPV) of STAT was 65%, while RB and ELISA had the same PPV (64%). On the other hand, the negative predictive values were 40%, 22% and 33% for STAT, RB and ELISA respectively. However, the specificity of both STAT and RB was surprisingly low (6%). On the other hand, ELISA had a sensitivity of 75% and a specificity of 23%. These results highlight the value of ELISA as a rapid and reliable tool for the diagnosis of brucellosis particularly in endemic areas

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Item type	Current library	Home library	Call number	Copy number	Status	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.11.19.Ph.D.2017.Sa.C (Browse shelf(Opens below))		Not for loan	01010110074578000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.11.19.Ph.D.2017.Sa.C (Browse shelf(Opens below))	74578.CD	Not for loan	01020110074578000

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Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

The aim of the study was to compare the performance of different serological tests in the diagnosis of brucellosis, considering quantitative real time PCR (qPCR) as the reference test. Quantitative real time PCR was positive for Brucella DNA in 65% out of 100 serum samples. Both the standard tube agglutination test (STAT) and rose bengal (RB) test revealed high sensitivity in the detection of brucellosis (95% and 89% respectively). The positive predictive value (PPV) of STAT was 65%, while RB and ELISA had the same PPV (64%). On the other hand, the negative predictive values were 40%, 22% and 33% for STAT, RB and ELISA respectively. However, the specificity of both STAT and RB was surprisingly low (6%). On the other hand, ELISA had a sensitivity of 75% and a specificity of 23%. These results highlight the value of ELISA as a rapid and reliable tool for the diagnosis of brucellosis particularly in endemic areas

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