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Detection of carbapenemase - producing enterobacteriaceae by the rapidec CARBA NP test / Marwa Mohammed Mohammed Ghobashy ; Supervised Amal Shafik Balbaa , Basma Ahmed Elawady

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Marwa Mohammed Mohammed Ghobashy , 2017Description: 104 P. : charts , facsimiles ; 25cmOther title:
  • الكشف عن البكتريا المعويه المنتجه لانزيم الكاربابيناميز عن طريق استخدام اختبار الرابيدك كاربا ان بي [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology Summary: Introduction: Rapid dissemination of carbapenem resistant Enterobacteriaceae represents a major threat to patient care and public health. Rapid detection and identification provides effective treatment and proper infection control measures. Aim of the work: The purpose of the study was to detect carbapenemase-producing Enterobacteriaceae with the Rapidec carba np test. Materials and methods: The study was conducted on 101 clinical samples. Sixty Enterobacteriaceae isolates were identified to species level by the API 20E then screened for carbapenemase-production by ertapenem disk diffusion method and Minimum inhibitory concentration methods. Confirmation was done by the Modified Hodge Test and the Rapidec carba np test. Results: Among the 60 Enterobacteriaceae isolates, E.coli was the most commonly encountered (51.6%), followed by K. pneumoniae (38.3%), K. oxytoca (3.3%), E. cloacae (1.6%) and P. mirabilis (5%). Out of the 60 isolates, 25 isolates (41.6%) were screened to be carbapenem resistant by ertapenem disk diffusion (ETP) and 35 isolates (58.3%) were sensitive. Twenty seven isolates (45%) were screened positive by MIC and 33 isolates (55%) were negative. Twenty five isolates (41.6%) were confirmed to be carbapenem resistant by the Modified Hodge test (MHT) and 35 isolates (58.3%) were sensitive. Twenty seven isolates (45%) were confirmed to be carbapenem resistant by the Rapidec carba np test and 33 isolates (55%) were sensitive. Conclusions: The Rapidec carba np test overall sensitivity was 100% and specificity was 94.29% in detecting carbapenemase producing Enterobacteriaceae isolates
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.M.Sc.2017.Ma.D (Browse shelf(Opens below)) Not for loan 01010110074764000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.M.Sc.2017.Ma.D (Browse shelf(Opens below)) 74764.CD Not for loan 01020110074764000

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

Introduction: Rapid dissemination of carbapenem resistant Enterobacteriaceae represents a major threat to patient care and public health. Rapid detection and identification provides effective treatment and proper infection control measures. Aim of the work: The purpose of the study was to detect carbapenemase-producing Enterobacteriaceae with the Rapidec carba np test. Materials and methods: The study was conducted on 101 clinical samples. Sixty Enterobacteriaceae isolates were identified to species level by the API 20E then screened for carbapenemase-production by ertapenem disk diffusion method and Minimum inhibitory concentration methods. Confirmation was done by the Modified Hodge Test and the Rapidec carba np test. Results: Among the 60 Enterobacteriaceae isolates, E.coli was the most commonly encountered (51.6%), followed by K. pneumoniae (38.3%), K. oxytoca (3.3%), E. cloacae (1.6%) and P. mirabilis (5%). Out of the 60 isolates, 25 isolates (41.6%) were screened to be carbapenem resistant by ertapenem disk diffusion (ETP) and 35 isolates (58.3%) were sensitive. Twenty seven isolates (45%) were screened positive by MIC and 33 isolates (55%) were negative. Twenty five isolates (41.6%) were confirmed to be carbapenem resistant by the Modified Hodge test (MHT) and 35 isolates (58.3%) were sensitive. Twenty seven isolates (45%) were confirmed to be carbapenem resistant by the Rapidec carba np test and 33 isolates (55%) were sensitive. Conclusions: The Rapidec carba np test overall sensitivity was 100% and specificity was 94.29% in detecting carbapenemase producing Enterobacteriaceae isolates

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