Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology

The influence of sun exposure, gravity, and years of facial muscle movements starts to appear as wrinkles on the skinHyaluronic acid injection can be used as a dermal filler to improve the skin's contour and reduce depressions in the skin.The present work aimed to examine the histopathological changes occurring in lower lip of the rats, as a result of HA injection and whether it could really bring us closer to the {u030F} ideal soft tissue filler material .̋ To fulfill this aim, lip sections of rats treated with HA at various intervals were histologically examined using (H&E stain), in addition to histochemical examination using (Masson{u2019}s Trichrome & Toluidine Blue stain) and immunohistochemical analysis, using CD68 antibody. The study was conducted on thirty-six adult male rats with age ranging from 2 to 3 months and weight ranging from 150-200g. They were divided into two groups, each consisting of 18 rats, the first group represents the control group I and the second group represents the experimental group II. Each group was subdivided into three subgroups, according to the time of sacrifice with intervals of 7 days, 45 days and 90 days following injection as group I A, B and C and group II A, B and C respectively. In experimental group II, each rat received 0.07 ml of HA filler, which was injected into the lower lip, whereas group I served as positive control, where the animals were injected with 0.9 % saline into the lower rat's lip in the same way as those of experimental group

Issued also as CD