Expression of environment stress resistance gene (S) in some poplar species / Amr Said Mohamed Said ; Supervised Mohamed Hassanein Soliman , Mona Hashem Ahmed Hussein , Mervat Mohamed Ali Gad

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture- Department of Genetics

This study was carried out to establish reliable techniques which can be used for introducing GRX-2 gene into Populus alba by using Agrobacterium tumefaciens during the period from 2012 to 2016 at the Biotechnology Research Lab., and Plant Tissue Culture Lab., Horticulture Research Institute, Agricultural Research Center, Egypt. The aims of this work were to optimization of an efficient transformation system for Populus alba plants involve the GRX-2 gene by using Agrobacterium tumefaciens mediated technique. This gene donates recipient plant salinity tolerance and subjecting P. alba transgenic plants to salinity stress to evaluate their stress tolerance. Genetic transformation of an elite white poplar genotype (Populus alba L.) was performed with vector pRI 101-ON DNA in Agrobacterium tumefaciens strain LBA4404 containing the Glutaredoxin-2 gene in order to enhance the resistance to abiotic harmful stress. The time period of shoot tips infection with Agrobacterium tumefaciens suspension culture (0, 5, 10, 20 and 30 min) was tested for the transformation frequency. The highest frequency of transformation was obtained with shoot tip explants infected with Agrobacterium culture for 20 minutes at the concentration of OD 600= 0.8. Isoenzymes peroxidase and polyphenyl oxidase were used as indicators for GRX-2 expression transgenic poplars had highly total peroxidase and polyphenyl oxidase expression compared with that of the control

Issued also as CD