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Association of variants in STAT5A & OLR1 with Egyptian buffalo milk properties / Abdelmonsif Ahmed Shawky Moaawad ; Supervised Sherif Mourad Sherif , Nermin Kamal Elhalawany

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Abdelmonsif Ahmed Shawky Moaawad , 2017Description: 67 , 25 P. : charts , facsimiles ; 25cmOther title:
  • مع خواص الحليب فى الجاموس المصري OLR1 & STAT5A ارتباط المتغيرات الجينية في كل من الجينات [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology Summary: In this work, we selected 19 SNPs that are located in 4 genes, STAT5A, OLR1, LF and TLR4 because of their previous contribution and association with milk production traits in cattle. The selected SNPs were genotyped on 74 multiparous Egyptian buffaloes via sequenom assay. Only 7 SNPs showed polymorphism, one of them located in intron 1 of OLR1 at position 423 with minor allele frequency (MAF) of 0.13. The other one lies in intron 1 of TLR4 at position 4525 that showed a MAF of 0.02. The remaining 5 SNPs are located in LF gene at positions -190, -472, -292, -19 and +35 from the transcription start site. Their MAF are: 0.13, 0.19, 0.18, 0.18 and 0.18, respectively. None of the polymorphic SNPs showed significant associations with milk yield of Egyptian buffalo what could be due to small sample size. Although no significant associations were detected between any of the investigated SNPs and milk yield in the current study, this study shades a light on some polymorphic sites in Egyptian buffalo that could be used as genetic markers. Hence, further association studies on these polymorphic sites in a larger sample size and against different traits are recommended
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2017.Ab.A (Browse shelf(Opens below)) Not for loan 01010110075541000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2017.Ab.A (Browse shelf(Opens below)) 75541.CD Not for loan 01020110075541000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology

In this work, we selected 19 SNPs that are located in 4 genes, STAT5A, OLR1, LF and TLR4 because of their previous contribution and association with milk production traits in cattle. The selected SNPs were genotyped on 74 multiparous Egyptian buffaloes via sequenom assay. Only 7 SNPs showed polymorphism, one of them located in intron 1 of OLR1 at position 423 with minor allele frequency (MAF) of 0.13. The other one lies in intron 1 of TLR4 at position 4525 that showed a MAF of 0.02. The remaining 5 SNPs are located in LF gene at positions -190, -472, -292, -19 and +35 from the transcription start site. Their MAF are: 0.13, 0.19, 0.18, 0.18 and 0.18, respectively. None of the polymorphic SNPs showed significant associations with milk yield of Egyptian buffalo what could be due to small sample size. Although no significant associations were detected between any of the investigated SNPs and milk yield in the current study, this study shades a light on some polymorphic sites in Egyptian buffalo that could be used as genetic markers. Hence, further association studies on these polymorphic sites in a larger sample size and against different traits are recommended

Issued also as CD

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