Efficacy of mesenchymal stem cell-derived microvesicles on wound healing in dogs / Omnia Mohammed Azzam Abdelsalam ; Supervised Omar Mohammed Salah Eldein Eltookhy , Ashraf Aly Eldesoky Shamaa , Gehan Gamil Ahmed Shehab

problems including wounds. The present study investigated whether MSCs derived microvesicles (MVs or Exosomes) can exert therapeutic effects on an experimental model of cutaneous injury and explored the underlying involving mechanisms. Nine dogs were used in this study. Dogs were randomly and equally allocated into 3 groups. Three bilateral full thickness circular wounds were created on the back of three groups of dogs using 2-cm dermal punch. The wounds were at least 2.5 cm apart. Saline was subcutaneously injected in 4 places around each wound area in group-I (control), whereas an equal volume of MSCs solution was similarly injected in group-II, and an equal volume of exosomal solution of MSCs derived MVs was similarly injected in group-III. Along the study, all dogs were evaluated through physical, histopathological and immunohistochemical assessments at the 3rd, 7th and 14th day after injection. The findings demonstrated that MSCs derived MVs had significantly promoted cutaneous wound healing, collagen synthesis, vascularization at wound sites and showed more rapid wound closure than the using of MSCs. The application of the exosomal solution had not only promoted the generation of newly formed vessels, but also have accelerated their development and maturation leading to a faster healing process. So we can conclude that the MSC-MVs appeared to be a superior candidate for treating cutaneous wounds than their originator cells

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Surgery Anesthesiology and Radiology

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