Effect of green tea extract and adipose stem cell therapy on experimentally induced diabetes type I keratopathy : A histological study / Dina Talat Diab ; Supervised Maha Baligh Zickri , Mohamed Hafez Ahmed , Hala Ahmed Elsherif

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Histology

The present work aimed at investigating and comparing the therapeutic effect of green tea extract (GTE) and adipose mesenchymal stem cells (AMSCs) individually or combined in induced diabetes type I keratopathy in adult male albino rats. Forty two adult male albino rats with average body weight 200 grams were divided into: Donor Group: 2 rats from which AMSCs were isolated. Group Ї: (Control Group) of 5 rats. Group II: (GTE Group): 7 rats were left for 4 weeks without therapy then were given GTE 50 mg/kg daily orally for 4 weeks. Group III: (Diabetic Group): 7 rats were injected intraperitoneal (IP) with STZ 50 mg/kg. Diabetes was confirmed by measuring the blood glucose level. The rats were left for 8 weeks without therapy. Group IV: (Diabetic and GTE Group): 7 rats injected with STZ and 4 weeks later, the rats were given GTE as in group II. Group V: (Diabetic and AMSCs Group): 7 rats injected with STZ and 4 weeks later, 1x106 rat AMSCs were injected into the tail vein, 0.5 ml twice with 24 hours interval. The rats were left for another 4 weeks without therapy. Group VI: (Diabetic, GTE and AMSCs Group): 7 rats injected with STZ and 4 weeks later, AMSCs were injected as in group V. Combined daily oral administration of GTE was performed as in groups II and IV. Animals of groups I, II, III, IV, V and VI were sacrificed 8 weeks from the start of the experiment. The eye specimens were subjected to histological, histochemical, immunohistochemical, morphometric and serological studies. Group III showed separation of the superficial layers of the corneal epithelium, lymphocytes at the epithelial surface, reduced thickness of the epithelium, a single layer of squamous epithelial cells, focal epithelial disruption and apparent focal loss of the epithelium overlying a blood vesseleed the most remarkable effect

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