The anti-proliferative and apoptotic induction effect of moringa oleifera extract on squamous cell carcinoma cell line : In vitro study / Sally Ibrahim Mohamed ; Supervised Heba Ahmed Farag , Dalia Hussein Elrouby , Olfat Gamil Shaker

Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology

Cancer has become a big threat to human population and a major cause of human mortality. In spite of advances in treatment modalities in targeted therapy, radiotherapy, chemotherapy and surgery, the prognosis of SCC is poor due to invasion, metastasis and recurrence. Moreover, chemotherapy and radiotherapy have many disastrous complications that{u2019}s why people all over the world are searching in alternative medicine for new treatment modalities. Moringa oleifera is a herb that has many uses in folk medicine. The present study was carried out to test the hypothesis that moringa oleifera leaf extract have antitumor activity. Laryngeal squamous cell carcinoma cell line (SCC15) was used in this work. It was purchased from VACSERA, Egypt and stored in liquid nitrogen containers at (-196{u00B0}C). The leaves of moringa oleifera (MO) were collected from the farm of Egyptian scientific society of moringa in national research center; Giza, Egypt. The collected leaves were air-dried, powdered and an ethanolic extract was prepared. The SCC cell line has been sub cultured and then treated with the MOE, incubated for 48 hours then MTT assay was carried out to determine the maximal inhibitory dose (IC50) according to which we carried a cell cycle analysis with different doses to determine the cytotoxic effect and apoptotic effect of the extract, Finally we carried Giemsa staining to observe the ability of the cells to form colonies using inverted phase microscope. The results of the MTT assay determined the MOE cytotoxicity, the results were in the form of the optical density (OD) through which viability percent was calculated. The higher viability percentage of SCC cell line was observed in the control group (100 %), the viability percent gradually decreased by increasing MOE concentration, to reach its lowest level at 100og/ml, where the viability percent reached 21.46%

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