header
Image from OpenLibrary

Evaluation of the role of microRNA 122a and Telomerase in hepatocellular carcinoma patients / Khaled Mabrouk Mohamad ; Supervised Nadida Abdelhamid Goher , Nihal Moustafa Elasaly , Walaa Ahmed Rabea

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Khaled Mabrouk Mohamad , 2017Description: 154 P. : charts , facsimiles ; 25cmOther title:
  • الحمض النووي الريبوزومي الصغير122 و التيلوميراز في مرضى سرطان الخلايا الكبدية [Added title page title]
Subject(s): Available additional physical forms:
  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Background: Hepatocellular carcinoma (HCC) is currently the fifth most common solid tumor worldwide and the third leading cause of cancer related death. miRNAs are 19- to 25-nucleotide-long RNAs, able to bind complementary sequences in 3{u2032}-untranslated regions (3{u2032}-UTR) of several target mRNAs to induce their degradation or translational repression. miR-122a, a hepato-specific miRNA, resulted down-regulated in the majority of HCCs and in all examined HCC-derived cell lines. The high frequency of aberrant regulation of these miRNAs in HCC versus non-tumor liver suggests that they might play an important role in hepatocarcinogenesis.Telomeres are specialized DNA{u2013}protein structures that cap the ends of linear chromosomes. Telomerase is a large ribonucleoprotein (RNP) complex that maintains telomeric DNA. Over 80% of human cancers show an activation of telomerase. Aim of the work: The present study was performed to evaluate the genetic role of miRNA 122a and Telomerase as reliable, non {u2013} invasive biochemical markers for early detection of hepato-cellular carcinoma Subjects and Methods: Analysis of the expression level of mature miRNA 122a (miR- 122a) and Telomerase in serum of 30 HCC patients ,30 HCV patients and 20 healthy subjects as control using quantitative reverse-transcription real time PCR (qRT-PCR). Results: Concerning miRNA-122a, the median fold change was statistically significant elevated in HCC patients when compared to both HCV and control groups. There was significant positive correlation between serum miRNA 122a and AST in HCV group. Regarding Telomerase,There was significant elevation in median fold change of serum Telomerase in HCC patients when compared to both HCV and control groups. A significant positive correlation was found between serum telomerase and total & direct bilirubin in HCC group. Conclusion: Current data suggest significant increase in miRNA 122a and Telomerase in serum of HCC patients
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2017.Kh.E (Browse shelf(Opens below)) Not for loan 01010110075934000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.07.Ph.D.2017.Kh.E (Browse shelf(Opens below)) 75934.CD Not for loan 01020110075934000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Background: Hepatocellular carcinoma (HCC) is currently the fifth most common solid tumor worldwide and the third leading cause of cancer related death. miRNAs are 19- to 25-nucleotide-long RNAs, able to bind complementary sequences in 3{u2032}-untranslated regions (3{u2032}-UTR) of several target mRNAs to induce their degradation or translational repression. miR-122a, a hepato-specific miRNA, resulted down-regulated in the majority of HCCs and in all examined HCC-derived cell lines. The high frequency of aberrant regulation of these miRNAs in HCC versus non-tumor liver suggests that they might play an important role in hepatocarcinogenesis.Telomeres are specialized DNA{u2013}protein structures that cap the ends of linear chromosomes. Telomerase is a large ribonucleoprotein (RNP) complex that maintains telomeric DNA. Over 80% of human cancers show an activation of telomerase. Aim of the work: The present study was performed to evaluate the genetic role of miRNA 122a and Telomerase as reliable, non {u2013} invasive biochemical markers for early detection of hepato-cellular carcinoma Subjects and Methods: Analysis of the expression level of mature miRNA 122a (miR- 122a) and Telomerase in serum of 30 HCC patients ,30 HCV patients and 20 healthy subjects as control using quantitative reverse-transcription real time PCR (qRT-PCR). Results: Concerning miRNA-122a, the median fold change was statistically significant elevated in HCC patients when compared to both HCV and control groups. There was significant positive correlation between serum miRNA 122a and AST in HCV group. Regarding Telomerase,There was significant elevation in median fold change of serum Telomerase in HCC patients when compared to both HCV and control groups. A significant positive correlation was found between serum telomerase and total & direct bilirubin in HCC group. Conclusion: Current data suggest significant increase in miRNA 122a and Telomerase in serum of HCC patients

Issued also as CD

There are no comments on this title.

to post a comment.