Biochemical studies of some plants as antioxidant and anticancer / Dalia Ahmed Hashim Mohamed ; Supervised Fouad Abdelrehim Ahmed , Amr Ahmed Mostafa , Nermien Zakaria Ahmed

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Biochemistry

Part one:This present study was designed to investigate the antioxidant activity of three ethanolic extracts of Annona squamosa (leaves),Coffea arabica (beans) and Moringa oleifera (leaves) In-vitro. 1,1-diphenyl-2-picryl-hydrazyl (DPPH), Hydroxyl radical scavenging (OH{u2022}), Ferric reducing antioxidant power (FRAP), Total antioxidant capacity (TAC),Catalase (CAT), and Peroxidise enzymes (POX). Total phenolic (TP), Total flavonoids (TF) and Total soluble protein (TSP) contents were determined. Also, the effects on lysosomal enzymatic activities of the three extracts and Rutin hydrate (standard) by two doses of each extract as compared to control group on the four marker lysosomal enzymatic activities namely: acid phosphatase "ACP"; Ý-galactosidase"Ý-GAL"; Ý-N-acetyl glucoseaminidase"Ý-NAG", and Ý-glucuronidase " Ý-GLU" after 60 and 120 minutes of incubation in rat liver were investigated.The chemical profile and the contents of each extract by HPLC method were also determined. Part two:The potential anti-proliferation properties of the three ethanolic extracts on human breast carcinoma cell line type (MCF-7) was investigated using neutral red uptake assay for the estimation of the cell viability.The results showed that, the most potent antioxidant activity was observed for Coffea arabica (beans) then Moringa oleifera (leaves) followed by Annona squamosa (leaves) and the most potent inhibitory effect on lysosomal enzymatic activities was observed for Moringa oleifera leaves) then Coffea arabica (beans) followed by Annona squamosa(leaves) as compared to Rutin hydrate which were dose and enzyme type dependent

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