Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

Granulocyte colony-stimulating factor (G-CSF) is a cytokine of great importance in clinical hematology and oncology. It stimulates proliferation and differentiation of neutrophil progenitors, which play important roles in host defense against infectious agents. Recombinant human G-CSF (rh-G-CSF) is available in two forms: a non-glycosylated form expressed in Escherichia coliandknown as Filgrastim, and a glycosylated form derived from Chinese hamster ovary cells (CHO) and known as Lenograstim. To elucidate the role of the sugar chain in the rh-G-CSF, some in vitro and in vivo comparative studies were performed. A bioassay based on the proliferation of murine myeloid cell line (NFS-60) was used for the in vitro comparative studies. Correspondingly, a prospective, randomized, open, crossover study in pediatric solid tumor was designed to investigate how the two products differ and the clinical implications for these differences, as well In an attempt to assess the anti-inflammatory properties of rh-G-CSF, lipopolysaccharide was used as an inflammatory stimulus in the murine myeloid cell line (NFS-60). The in vitro studies suggested that Lenograstim was more than 26% more potent than Filgrastim

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