Molecular characterization of Hydatid cysts collected from different intermediate hosts / Sohila Mohamed Elgameel Aboelala ; Supervised Waheed M. Ali Mousa , Olfat A. Mahdy , Azza M. Abdelwahab

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Parasitology

Cystic echinococcosis is an important cosmopolitan parasitic zoonosis which causes a public health and economic problems in Egypt. So the incidence of Hydatid cyst (HC) infection was detected during the period from May 2015 to April 2016. It was found to be 7.5% in camels, 0.3% in cattle, 0.5% in sheep and 6.5% in donkeys. The SDS-PAGE analysis of camel crude HC fluid (cHCF) antigen gave 5 bands of molecular weights 181.0, 83.0, 70.0, 63.0 and 38.0 KDa. While partially purified HC fluid (ppHCF) Ag fractionated into 8 bands at molecular weight of 181.0, 83.0, 70.0, 63.0, 58.0, 48.0, 38.0 and 18.0 KDa. In addition to that, protoscolices (PSC) Ag separated into 4 bands at 181.0, 83.0, 70.0 and 63.0 KDa. Protective immunity against secondary hydatidosis was induced using three HC antigens (camel origin); cHCF, ppHCF and PSC antigens then challenged intra peritoneally with 2000 viable protoscolices. Humeral immune response accompany this protection was evaluated using ELISA. Percent of protection against HC was 86.0 %, 93.0 % and 88.4 % in immunized rabbits with cHCF, ppHCF and PSC antigens respectively. Antibody level in immunized group with ppHCF antigen on day 28 was higher than before immunization and was higher than that in PSC and cHCF antigen groups. This result indicated that ppHCF antigen can be used as a candidate for vaccine production. Also in this study germinal layers of 8 HC samples (3 of camels, 1 of cattle, 1 of sheep and 3 of donkeys) in addition to 3 secondary HC collected from rabbits were used as a source of DNA. PCR amplification of ITS1 gene of all extracted DNA samples showed an amplified DNA band of the same molecular size at 1115 bp on agarose gel

Issued also as CD