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Serological and molecular diagnosis of campylobacter of poultry origin / Mai Mahmoud Morsy Ahmed ; Supervised Mona Ibrahim Hassan Elenbaawy , Soad Abdelaziz Elwanis

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mai Mahmoud Morsy Ahmed , 2018Description: 244 P. : charts ; 25cmOther title:
  • التشخيص السيرولوجي والجزيئى للكامبيلوباكتر ذات المصدر الداجني [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: Two hundred and nineteen samples were collected from poultry (chicken, pigeon, duck and turkey). Pigeon samples showed highest percentage of isolation with percentage of 27% followed by chicken with percentage of 23% while turkey showed 0%. The predominant isolates of the chicken were identified as C.coli with percentage of 55.6%while the C.jejuni was the most predominant isolates in pigeon isolates with the percentage of 58.8% and two isolates were untyped by culture methods. The highest percentage of isolation was recorded in the summer with a percentage of 35.7% followed by winter with a percentage of 27.4%, and then autumn with percentage of 14% and 0% was recorded in the spring. This study aimed to compare Campylobacter spp. detection by molecular method (multiplex real time polymerase chain reaction) and Ag capture ELISA to the culture method, 76 samples were selected from previous collected samples depended on case history. Out of 76 samples tested (27.6%) were positive by culture method, (85.5%) were positive by Ag capture ELISA and 76.3% were positive by multiplex real time PCR. The Campylobacter spp. identified by culture method were 10 (45.5%) C. jejuni and 12 (54.4%) C. coli while multiplex real time PCR was identified 33 (56.9%) C. coli, 17(29.3%) C.jejuni and 4 (6.1%) as mixed C.jejuni and C.coli
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.Ph.D.2018.Ma.S (Browse shelf(Opens below)) Not for loan 01010110076520000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.Ph.D.2018.Ma.S (Browse shelf(Opens below)) 76520.CD Not for loan 01020110076520000

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Two hundred and nineteen samples were collected from poultry (chicken, pigeon, duck and turkey). Pigeon samples showed highest percentage of isolation with percentage of 27% followed by chicken with percentage of 23% while turkey showed 0%. The predominant isolates of the chicken were identified as C.coli with percentage of 55.6%while the C.jejuni was the most predominant isolates in pigeon isolates with the percentage of 58.8% and two isolates were untyped by culture methods. The highest percentage of isolation was recorded in the summer with a percentage of 35.7% followed by winter with a percentage of 27.4%, and then autumn with percentage of 14% and 0% was recorded in the spring. This study aimed to compare Campylobacter spp. detection by molecular method (multiplex real time polymerase chain reaction) and Ag capture ELISA to the culture method, 76 samples were selected from previous collected samples depended on case history. Out of 76 samples tested (27.6%) were positive by culture method, (85.5%) were positive by Ag capture ELISA and 76.3% were positive by multiplex real time PCR. The Campylobacter spp. identified by culture method were 10 (45.5%) C. jejuni and 12 (54.4%) C. coli while multiplex real time PCR was identified 33 (56.9%) C. coli, 17(29.3%) C.jejuni and 4 (6.1%) as mixed C.jejuni and C.coli

Issued also as CD

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