Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology

Salivary gland neoplasms have a special status in human neoplasia, as they have the most complex histopathology of any tissue, with an exceptional breadth of different tumour types that exhibit a wide variety of microscopic appearances. The treatment options for salivary gland cancer depend largely on the stage of the cancer. In the past few years, the emergence of induced pluripotent stem cells (IPS) has boosted the stem cell field. We aimed to generate IPS cells to be used as a treatment modality for induced salivary gland cancer and to examine its effect as a in restoring salivary gland function by detection of RNA concentration and the count of acinar cells. Forty two white male albino rats were used and the induction done at their submandibular glands using squamous cell carcinoma cell lines (SCC). All the submandibular glands from rats were examined clinically and histologically to detect cancer formation. They revealed criteria of malignancy for SCC. IPS treated cases revealed high therapeutic regeneration of salivary glands as they regained normal architecture of acinar pattern that resemble normal tissues to a great extent. Moreover, minor degenerative changes and vaculaization still present. The acinar cells regained their proper organization with maintenance of abnormal activity such as hyperchromatism. Statistical analysis revealed highly significant difference among the experimental groups for PCR among different genes (P {u02C2}0.05*) and acinar quantification control (268.8±13.58), SCC (120.8±14.52) and IPS (197.8±9.94) (0.00001**). We concluded that IPS cells might represent an effective therapeutic modality for salivary gland carcinomas

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