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Molecular characterization of Eimeria tenella infecting chicken in Egypt / Hend Monir Hassan Mohamed ; Supervised Waheed M. Ali Mousa , Sahar Z. Abdelmawgood , Salwa M. Awadalla

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Hend Monir Hassan Mohamed, 2018Description: 114 P. : facsimiles ; 25cmOther title:
  • التمييز الجزيئي للايميريا تينيلا التى تصيب الدجاج في مصر [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Parasitology Summary: Coccidiosis in chickens is one of the major problems of poultry industry that is caused by protozoan parasites of genus Eimeria. Cecal contents and cecal mucosal scraping samples were collected from 50 naturally infected native broiler chicken aging 25 days. The post mortem examination showed hemorrhagic mucosa with bloody cecal core in two cecai. Eimeria oocysts were isolated by single oocyst technique. The obtained sporulated oocysts were identified according to morphological features, and the calculated mean oocyst shape index of randomly 50 oocysts (1.22 om), mean length (25.2 om) with SD (±2.24 om), and mean width (20.6 om) with SD (±1.6om) as E. tenella. Species-specific polymerase chain reaction (PCR) test targeting the internal transcribed spacer-1 (ITS-1) sequences of the genomic rDNA using primer pairs (E. tenella ET, E. maxima Ema and Emu and E. nexatrix EN) was performed for isolated Eimeria species. The results of species-specific PCR assays confirmed the presence of E. tenella as it has the characteristic in all of the tested samples bp of 278 at common optimum annealing temperature for this species was determined to be 58 {u00B0}C but it didn{u2019}t show any band with other previous mentioned primer pairs
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.12.M.Sc.2018.He.M (Browse shelf(Opens below)) Not for loan 01010110076768000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.12.M.Sc.2018.He.M (Browse shelf(Opens below)) 76768.CD Not for loan 01020110076768000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Parasitology

Coccidiosis in chickens is one of the major problems of poultry industry that is caused by protozoan parasites of genus Eimeria. Cecal contents and cecal mucosal scraping samples were collected from 50 naturally infected native broiler chicken aging 25 days. The post mortem examination showed hemorrhagic mucosa with bloody cecal core in two cecai. Eimeria oocysts were isolated by single oocyst technique. The obtained sporulated oocysts were identified according to morphological features, and the calculated mean oocyst shape index of randomly 50 oocysts (1.22 om), mean length (25.2 om) with SD (±2.24 om), and mean width (20.6 om) with SD (±1.6om) as E. tenella. Species-specific polymerase chain reaction (PCR) test targeting the internal transcribed spacer-1 (ITS-1) sequences of the genomic rDNA using primer pairs (E. tenella ET, E. maxima Ema and Emu and E. nexatrix EN) was performed for isolated Eimeria species. The results of species-specific PCR assays confirmed the presence of E. tenella as it has the characteristic in all of the tested samples bp of 278 at common optimum annealing temperature for this species was determined to be 58 {u00B0}C but it didn{u2019}t show any band with other previous mentioned primer pairs

Issued also as CD

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