Evaluation of the antitumor effect of crude Cobra venom in mice with reference to clinicopathological changes / Mohamed Adel Ibrahim ; Supervised Amira Hassan Mohamed , Nashwa Adel Abuaita , Aly Fahmy Mohamed

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Clinical Pathology

Snake venom is composed of different proteins and enzymes which have been shown to exert beneficial effects in treatment of certain diseases due to its various biological activities. The present study was divided into two experiments. Experiment one aimed to evaluate the clinicopathological effect of Naja haje snake venom at different doses on hematological, serum biochemical parameters, oxidative damage and histopathological alterations in mice. Results showed that administration of 2.1æg/ml venom caused anemia, leukopenia, hypoproteinemia, hypoalbuminemia, decreased A/G ratio and hepatic and renal GSH, while there were significant increases in the value of total and conjugated bilirubin, activities of ALT, AST and ALP, values of serum urea, creatinine and hepatic and renal MDA levels. Snake venom caused histopathological changes in the liver, kidney and spleen of mice. Experiment two was conducted to evaluate the antitumor effect of Naja haje snake venom by I/P injection of EAC tumor cells together with snake venom in different concentrations. Group of mice injected with tumor cells alone showed anemia, significant hypoproteinemia, hypoalbuminemia decreased A/G ratio, decrease of hepatic and renal GSH, increase of total leukocytic counts and platelet counts, values of total and conjugated bilirubin, activities of ALT, AST and ALP, values of serum urea, creatinine and hepatic and renal MDA levels. Values of the tested parameters were brought back to near normal levels in groups treated with different concentrations of snake venom

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