Evaluation of the osteogenic potential of zoledronic acid and / or recombinant parathormone hormone (1-34) on glucocorticoid induced osteoporosis in rats : Animal study / Israa Ahmed Gamal Eldin Radwan ; Supervised Nahed Sedky Korany , Bassant Adel Ezzat
Material type: TextLanguage: English Publication details: Cairo : Israa Ahmed Gamal Eldin Radwan , 2018Description: 185 P. : facsimiles ; 25cmOther title:- تقييم إمكانات التكوين العظمي لحمض الزوليدرونك و/ أو هرمون باراثورمون المؤتلف على هشاشة العظام المسببه بمادة الجلايكورتيكود فى الفئران : دراسه داخل الحيوان [Added title page title]
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Item type | Current library | Home library | Call number | Copy number | Status | Date due | Barcode | |
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Thesis | قاعة الرسائل الجامعية - الدور الاول | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.09.12.Ph.D.2018.Is.E (Browse shelf(Opens below)) | Not for loan | 01010110076820000 | |||
CD - Rom | مخـــزن الرســائل الجـــامعية - البدروم | المكتبة المركزبة الجديدة - جامعة القاهرة | Cai01.09.12.Ph.D.2018.Is.E (Browse shelf(Opens below)) | 76820.CD | Not for loan | 01020110076820000 |
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Thesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral Biology
The present study was conducted to evaluate and compare between the effect of using parathyroid hormone 1-34 and zoledronic acid, separately or concomitantly on microarchitecture, alkaline phosphatase activity of alveolar process and bone mineral density of rats{u2019} mandible with glucocorticoid (GCs) induced osteoporosis via histology, histomorphometry, dual energy x-ray absorptiometry (DXA) scan, scanning electron microscope (SEM), bone morphomety, and alkaline phosphatase enzymatic assay analysis. A total of 146 adult female albino rats were used in the current study. All rats received a weekly dose 7 mg/kg body weight dexamethasone for 5 weeks. This was followed by scarification of 2 rats to assure osteoporosis induction. 144 rats were randomly distributed into four groups: Control group (36 animals): received daily S.C. injection of saline. Aclasta group (36 animals): received weekly S.C. injection of 7.5 {u1D707}g/kg body weight Aclasta. Forteo group (36 animals): received daily S.C. injection of 60 og/kg Forteo. Combination group (36 animals): received weekly S.C. injection of 7.5 {u1D707}g/kg body weight Aclasta plus daily S.C. injection of 60 og/kg Forteo. The animals were sacrificed at 2 and 4 weeks after initiation of drug administration. The mandible was retrieved, then divided into two halves. The hemi-mandibles were used as following: The right halves of mandibles of 96 rats (n = 96, 24 per group and 12 per time) were fixed in 10% calcium formol solution for 48 hours, before examining using DXA scanner for bone mineral density assessment. After that, hemi-mandibles were processed and the alveolar bone surrounding the roots of the first molar was examined through routine histological and histomorphometric evaluation
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