Study of the possible cardio-renal protective effect of some drugs in cyclophosphamide treated rats / Khaled Ali Abdo Alhumaidha ; Supervised Helmy Moawad Sayed Ahmed , Wafaa Ibrahim Eleraky , May Ahmed Galal Abdelfattah

Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology

Cyclophosphamide (CP) is a cytotoxic alkylating agent used in the treatment of malignant diseases and autoimmune disorders. Its clinical use is limited to its marked cardiorenal toxicity. The present study aimed to investigate the possible protective role of taurine (Tau; 200 mg/kg) and pravastatin (Prv; 10 mg/kg) on CP-induced cardiorenal toxicity. CP (200 mg/kg) was administered as a single intraperitoneal injection whereas, Tau, Prv and their combination were administered for three weeks on a daily basis. Blood and tissue samples were collected for different biochemical and histological studies for both cardiac and renal tissues.The results of the present study showed that CP produced an elevation in serum activities of creatine kinase, creatine kinase isoenzyme, lactate dehydrogenase, creatinine as well as blood urea nitrogen. Cyclophosphamide also induced an elevation in the oxidative stress markers, as evidenced by elevation in lipid peroxides content (measured as malondialdehyde; MDA) and reduction in reduced glutathione and superoxide dismutase activity in both cardiac and renal tissues. On the other hand, administration of Tau or Prv attenuated the CP-evoked disturbances in the above mentioned parameters. In addition, CP exhibited electrocardiographic changes, which were significantly reversed by Tau and Prv treatment. Histopathological examination of both cardiac and renal tissues showed that CP-caused significant structural damages that were reduced by Tau and Prv. From all the previous results, it could be concluded that both Tau and Prv have a potentiality to ameliorate CP-induced cardiorenal toxicity due to their antioxidant activity

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