Characterization of antimicrobial resistance determinants of salmonella / Alaa Ahmed Elgabaly Mostafa Ahmed ; Supervised Nashwa Abdelsalam Ezz Eldeen , Ahmed Samir Mohamed Shehatta , Mohamed Abdalla Abdelmonem

By:

Alaa Ahmed Elgabaly Mostafa Ahmed

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Cairo : Alaa Ahmed Elgabaly Mostafa Ahmed , 2018Description: 121 P. : charts , facsimiles ; 25cmOther title:

توصيف محددات المقاومة الميكروبية علي بكتريا السالمونيلا [Added title page title]

Subject(s):

Available additional physical forms:

Issued also as CD

Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: Salmonellosis is one of the most common causes of food-borne disease worldwide. Most infections are zoonotic and are transmitted from food animals to humans through the ingestion of contaminated food. The present investigation was carried out to evaluate the prevalence of resistance genes in multi-drug resistant and ESÝL Salmonella isolates, knowing identity between Salmonella isolates from different sources, identifying the best choice of antibiotics according to CLSI of Enterobacteriaceae, identifying another choice of treatment as hibiscus extracts by different solvents as the strains were MRD and applying the extract in vivo to confirm its efficiency. The result of antibiotics sensitivity indicated that the recovered Salmonella isolates were highly resistance to Amikacin , Trimethoprim +Sulfamethoxazole, Amoxicillin+ Clavulanic acid, Streptomycin, Chloramphenicol, Tetracycline, cephalothin, Ciprofloxacin, Ampicillin, Gentamicin, Amoxicillin and Norfloxacin with percentage 100%.While On the other hand all tested isolates were sensitive to Ceftazidime and Imipenem.. ESÝL test applied to the isolates and the results showed that 78.9 % of isolates were positive ESÝL.PCR was designed to detect blaTEM (Ý-lactamases), TET (A) (Tetracycline), floR (Chloramphenicol), gyr(A) and Sul1 (Sulphonamides) genes in Salmonella resistant isolates.Testing for the resistance genes using PCR showed that 100% of the tested isolates were positive to gyrA, Sul1and TET(A) gene, while 78.9% of the tested isolates were positive to floR gene and finally 71% of the tested isolates were positive to blaTEM gene

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.10.Ph.D.2018.Al.C (Browse shelf(Opens below))		Not for loan		01010110076882000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.10.10.Ph.D.2018.Al.C (Browse shelf(Opens below))	76882.CD	Not for loan		01020110076882000

Browsing المكتبة المركزبة الجديدة - جامعة القاهرة shelves Close shelf browser (Hides shelf browser)

Previous	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	Next
Previous	Cai01.10.10.Ph.D.2018.Ah.S Sources of bacterial contamination in slaughterhouses with special reference to the methods of their control /	Cai01.10.10.Ph.D.2018.Ah.S Sources of bacterial contamination in slaughterhouses with special reference to the methods of their control /	Cai01.10.10.Ph.D.2018.Al.C Characterization of antimicrobial resistance determinants of salmonella /	Cai01.10.10.Ph.D.2018.Al.C Characterization of antimicrobial resistance determinants of salmonella /	Cai01.10.10.Ph.D.2018.Al.I Immunological studies of C. perfringens type "A" using nanotechnology /	Cai01.10.10.Ph.D.2018.Al.I Immunological studies of C. perfringens type "A" using nanotechnology /	Cai01.10.10.Ph.D.2018.Es.A Attemptto prepare Chlamydia psittaci Vaccine /	Next

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Salmonellosis is one of the most common causes of food-borne disease worldwide. Most infections are zoonotic and are transmitted from food animals to humans through the ingestion of contaminated food. The present investigation was carried out to evaluate the prevalence of resistance genes in multi-drug resistant and ESÝL Salmonella isolates, knowing identity between Salmonella isolates from different sources, identifying the best choice of antibiotics according to CLSI of Enterobacteriaceae, identifying another choice of treatment as hibiscus extracts by different solvents as the strains were MRD and applying the extract in vivo to confirm its efficiency. The result of antibiotics sensitivity indicated that the recovered Salmonella isolates were highly resistance to Amikacin , Trimethoprim +Sulfamethoxazole, Amoxicillin+ Clavulanic acid, Streptomycin, Chloramphenicol, Tetracycline, cephalothin, Ciprofloxacin, Ampicillin, Gentamicin, Amoxicillin and Norfloxacin with percentage 100%.While On the other hand all tested isolates were sensitive to Ceftazidime and Imipenem.. ESÝL test applied to the isolates and the results showed that 78.9 % of isolates were positive ESÝL.PCR was designed to detect blaTEM (Ý-lactamases), TET (A) (Tetracycline), floR (Chloramphenicol), gyr(A) and Sul1 (Sulphonamides) genes in Salmonella resistant isolates.Testing for the resistance genes using PCR showed that 100% of the tested isolates were positive to gyrA, Sul1and TET(A) gene, while 78.9% of the tested isolates were positive to floR gene and finally 71% of the tested isolates were positive to blaTEM gene

Issued also as CD

There are no comments on this title.

to post a comment.