Thesis (M.Sc.) - Cairo University - Institute of African Research and Studies - Department of Natural Resources

This study was carried out in coordination with Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Center, Cairo, Egypt, and Plant Resources Laboratory, Natural Resources Department, Institute of African Research and Studies, Cairo University, during the period from 2014 to 2017. This study includes application of different techniques of plant biotechnology and biochemistry, i.e. breaking of seed dormancy, establishment and production of callus from different explants of African locust bean plant, in vitro production of cell suspension from callus tissue. Studies on the effect of salinity on cell suspension cultures, molecular characterization and isozyme techniques of salt treated cell suspension cultures compared with callus cells, quantitative determination of total phenol compounds of treated cell suspension culture and callus cell culture, separation and identification of phenolic compound using HPLC technique. The obtained result revealed that using concentrated H2SO4 (98%) for 5 min recorded the best result, for breaking seed dormancy with maximum germination percentage (100%) and seedlings growth. Moreover, in establishment stage for callus production, sterilization of seeds by clorox (sodium hypochloride 5.25%) 60% for 25 min recorded the survival rate 100 % and no contamination. Callus production from stem explants was observed in medium contain 1 mg/l 2,4-D. MS nutrient medium gave the highest production of callus from stem explants as compared with other media

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