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Preparation and evaluation of a combined bivalent vaccine against avian pasteurella and mycoplasma infections in chickens / Fatma Fathy Ibrahim Hassan ; Supervised Mahmoud Essam Hatem Ahmed , Jakeen Kamal Eljakee , Wafaa Abdelghany Abdelghany

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Fatma Fathy Ibrahim Hassan , 2018Description: 136 P. ; 25cmOther title:
  • تحضير وتقييم لقاح مشترك ثنائى ضد عدوى الباستريلا والميكوبلازما فى الدجاج [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: The present work was planned to study the immune response of chickens vaccinated with locally prepared combined inactivated vaccine of M. gallisepticum and P. multocida adjuvanted with Montanide ISA70. 150, 4 weeks old specific pathogen free chickens were divided into five groups, the 1st group was vaccinated with P. multocida vaccine, the 2nd group was vaccinated with M. gallisepticum vaccine, the 3rd group was vaccinated with combined M. gallisepticum and P. multocida vaccine, the 4th group was vaccinated with imported M. gallisepticum vaccine and the 5th group was kept unvaccinated as a control group. The prepared vaccines were evaluated by determination of the cellular immunity by heterophils/lymphocytes ratio and measurement of nitric oxide in the supernatant of macrophage, and evaluation of the humoral immunity by indirect haemagglutination, haemagglutination inhibition and ELISA techniques.The potency of the vaccines were evaluated by the challenge and passive mouse protection tests against the challenge with the virulent strain of M. gallisepticum (Eis3-10 strain) and P. multocida (serotypes A and D). The results showed that combined inactivated vaccine of M. gallisepticum and P. multocida adjuvanted with Montanide ISA70 induced high and long duration of antibody response and significant protection against the challenge with virulent strain of M. gallisepticum (Eis3-10 strain)
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.Ph.D.2018.Fa.P (Browse shelf(Opens below)) Not for loan 01010110077136000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.Ph.D.2018.Fa.P (Browse shelf(Opens below)) 77136.CD Not for loan 01020110077136000

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

The present work was planned to study the immune response of chickens vaccinated with locally prepared combined inactivated vaccine of M. gallisepticum and P. multocida adjuvanted with Montanide ISA70. 150, 4 weeks old specific pathogen free chickens were divided into five groups, the 1st group was vaccinated with P. multocida vaccine, the 2nd group was vaccinated with M. gallisepticum vaccine, the 3rd group was vaccinated with combined M. gallisepticum and P. multocida vaccine, the 4th group was vaccinated with imported M. gallisepticum vaccine and the 5th group was kept unvaccinated as a control group. The prepared vaccines were evaluated by determination of the cellular immunity by heterophils/lymphocytes ratio and measurement of nitric oxide in the supernatant of macrophage, and evaluation of the humoral immunity by indirect haemagglutination, haemagglutination inhibition and ELISA techniques.The potency of the vaccines were evaluated by the challenge and passive mouse protection tests against the challenge with the virulent strain of M. gallisepticum (Eis3-10 strain) and P. multocida (serotypes A and D). The results showed that combined inactivated vaccine of M. gallisepticum and P. multocida adjuvanted with Montanide ISA70 induced high and long duration of antibody response and significant protection against the challenge with virulent strain of M. gallisepticum (Eis3-10 strain)

Issued also as CD

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