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Rapid identification of pasteurella multocida isolates recovered from small and large ruminants / Adel Mostafa Abdelrahman Mostafa ; Supervised Mohamed Kamal Refai , Sherif Abdel monem Maerouf , Sahar Roshdy Mohamed

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Adel Mostafa Abdelrahman Mostafa , 2018Description: 141 P. : charts , facsimiles ; 25cmOther title:
  • التصنيف السريع لعترات الباستريلا مالتوسيدا المعزوله من المجترات الصغيره و الكبيره [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: Pasteurella multocida is the common cause of respiratory disease in small (sheep and goat) and large (cattle and buffalo) ruminants causing high economic losses. A total of 36 P. multocida isolates were detected from 451 samples (8%). Lung tissues showed the highest percentage of Pasteurella multocida isolation (23/189) as a percentage (12%), followed by nasal swabs (13/262) as a percentage (4.9%). These isolates were confirmed microscopically, biochemically and rabidly by using Vitek2 compact system. PMT-ELISA and mouse lethality test was used for differentiation between toxigenic and non-toxigenic isolates. Polymerase Chain Reaction (PCR) system was used for detection of tox A gene and multiplex PCR was used for capsular serotyping of toxigenic isolates .A total of 11 isolates showing to be toxigenic by mouse lethality test a percentage (31%) and 16 isolate showing positive to tox A by using PMT-ELISA a percentage (44.4%).PCR was used to detect tox A gene and showed that 16 isolates were positive for tox A gene. Multiplex PCR by using amplified DNA showed that all toxigenic isolates of P. multocida were capsular type A. DNA nucleotide sequencing and analysis of sequences obtained for the tox A gene under investigation observed that: P. multocida isolates had no genetic diversity for tox A gene among small and large ruminants and the identity between 2 isolate 99.6% to 100%
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2018.Ad.R (Browse shelf(Opens below)) Not for loan 01010110077140000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2018.Ad.R (Browse shelf(Opens below)) 77140.CD Not for loan 01020110077140000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Pasteurella multocida is the common cause of respiratory disease in small (sheep and goat) and large (cattle and buffalo) ruminants causing high economic losses. A total of 36 P. multocida isolates were detected from 451 samples (8%). Lung tissues showed the highest percentage of Pasteurella multocida isolation (23/189) as a percentage (12%), followed by nasal swabs (13/262) as a percentage (4.9%). These isolates were confirmed microscopically, biochemically and rabidly by using Vitek2 compact system. PMT-ELISA and mouse lethality test was used for differentiation between toxigenic and non-toxigenic isolates. Polymerase Chain Reaction (PCR) system was used for detection of tox A gene and multiplex PCR was used for capsular serotyping of toxigenic isolates .A total of 11 isolates showing to be toxigenic by mouse lethality test a percentage (31%) and 16 isolate showing positive to tox A by using PMT-ELISA a percentage (44.4%).PCR was used to detect tox A gene and showed that 16 isolates were positive for tox A gene. Multiplex PCR by using amplified DNA showed that all toxigenic isolates of P. multocida were capsular type A. DNA nucleotide sequencing and analysis of sequences obtained for the tox A gene under investigation observed that: P. multocida isolates had no genetic diversity for tox A gene among small and large ruminants and the identity between 2 isolate 99.6% to 100%

Issued also as CD

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