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Comparative bacteriological and molecular studies on klebsiella species isolated from chicken and human / Ahmed Mohammed Emam ; Supervised Heidy Mohamed Shawky , Ahmed Samir Mohamed , Ebtesam Mohamed Mahmoud

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Ahmed Mohammed Emam , 2018Description: 127 P. : charts , facsimiles ; 25cmOther title:
  • دراسات بكتريولوجية وجزيئية مقارنة على أنواع الكليبسيللا المعزولة من الدجاج والانسان [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology Summary: In the present study,1000 samples (liver, spleen, lung and trachea) from apparently healthy and diseased broiler, layer and baby chicks and 200 human samples bronchoalveolar lavage BAL, blood stream infection BSI, urine UR, wound W tissue T were collected to investigate the prevalence of Klebsiella spp. The isolation of Klebsiella from broilers revealed 4 isolates (5.8%), 8 isolates(5.3%) from layers, 3isolates from baby chicks (9.6%) and 16 human isolates (7.5%).Antimicrobial sensitivity testing against16 antibiotic agents revealed that all of isolates (100%) were sensitive to amikacin, Cefepime and imipenem followed by (97.3%) gentamicin, (94.6%) aztreonam and (86.5%) ciprofloxacin. While, (97.3%) of isolates were resistant to nalidixic acid followed by (94.6%) resistant to ampicillin, (83.8%) to tetracycline and (70.3%) to sulphamethoxazole/trimethoprim. Serological survey using; Molecular characterization of some ESBLs genes (blaTEM, blaCTX and blaSHV) using PCR showed that 6 isolates were positive for blaTEM gene and 5were positive for blaCTX gene. All isolates were negative for blaSHV gene
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2018.Ah.C (Browse shelf(Opens below)) Not for loan 01010110077306000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.10.M.Sc.2018.Ah.C (Browse shelf(Opens below)) 77306.CD Not for loan 01020110077306000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

In the present study,1000 samples (liver, spleen, lung and trachea) from apparently healthy and diseased broiler, layer and baby chicks and 200 human samples bronchoalveolar lavage BAL, blood stream infection BSI, urine UR, wound W tissue T were collected to investigate the prevalence of Klebsiella spp. The isolation of Klebsiella from broilers revealed 4 isolates (5.8%), 8 isolates(5.3%) from layers, 3isolates from baby chicks (9.6%) and 16 human isolates (7.5%).Antimicrobial sensitivity testing against16 antibiotic agents revealed that all of isolates (100%) were sensitive to amikacin, Cefepime and imipenem followed by (97.3%) gentamicin, (94.6%) aztreonam and (86.5%) ciprofloxacin. While, (97.3%) of isolates were resistant to nalidixic acid followed by (94.6%) resistant to ampicillin, (83.8%) to tetracycline and (70.3%) to sulphamethoxazole/trimethoprim. Serological survey using; Molecular characterization of some ESBLs genes (blaTEM, blaCTX and blaSHV) using PCR showed that 6 isolates were positive for blaTEM gene and 5were positive for blaCTX gene. All isolates were negative for blaSHV gene

Issued also as CD

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